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MABT1481-25UG Anti-COL4A6 Antibody, clone 122-2

Anti-COL4A6, clone 122-2, Cat. No. MABT1481, rat monoclonal antibody that detects Collagen type IV 6 chain (COL4A6) and is tested for use in ELISA, Immunohistochemistry, and Western Blotting.

MABT1481-25UG
25 μg  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberMABT1481-25UG
      DescriptionAnti-COL4A6 Antibody, clone 122-2
      Alternate Names
      • Collagen type IV alpha 6 chain
      Background InformationCollagen type IV alpha 6 chain (UniProt: A0A0G2K601; also known as Col4a6) is encoded by the Col4a6 gene (Gene ID: 363458) in rat. The collagen super family includes over 20 different types of collagens with at least 38 distinct polypeptide chains. Of these types I, II, and III are the most abundant and are involved in the formation of fibrils. Type IV collagen forms a two-dimensional reticulum and is a major component of the basal lamina. It is a network-forming collagen that underlies epithelial and endothelial cells and functions as a barrier between tissue compartments. Type IV collagen has many binding partners and forms the backbone of the basement membrane. It also contains numerous cysteine residues that are involved in inter- and intramolecular disulfide bonding. The type IV collagen family comprises six isomeric chains, designated 1(IV) to 6(IV), each of which can form a triple helix structure with 2 other chains to generate type IV collagen network. Each type of collagen IV is a heterotrimer composed of three chains. Formation of these heterotrimers is initiated by the non-collagenous 1 (NC1) domain interactions and is accompanied by folding of the collagenous domains into triple helices. Collagen IV 6 is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to generate the mature form. Clone 122-2 specifically recognizes collagen IV 6 and reacts with the NC1 domain. (Ref.: Namba, M., et al. (2021). Sci. Rep. 11; 20836; Khoshnoodi, J., et al. (2008). Microsc. Res. Tech. 71(5); 357-370).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-COL4A6, clone 122-2, Cat. No. MABT1481, rat monoclonal antibody that detects Collagen type IV 6 chain (COL4A6) and is tested for use in ELISA, Immunohistochemistry, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • ELISA
      Application NotesTested Applications

      Immunohistochemistry Applications: A representative lot detected COL4A6 in Immunohistochemistry applications (Namba, M., et al. (2021). Sci Rep. 11(1):20836).

      Western Blotting Analysis: A representative lot detected Col4a6 in Western Blotting applications (Namba, M., et al. (2021). Sci Rep. 11(1):20836).

      ELISA Analysis: A representative lot detected Col4a6 in ELISA applications (Namba, M., et al. (2021). Sci Rep.11(1):20836).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 231 amino acids from the C-terminal region of rat Collagen type IV 6 chain (COL4A6). It was injected into Col4a5 deficient male rats.
      EpitopeC-terminal
      Clone122-2
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRat
      SpecificityClone 122-2 is a rat monoclonal antibody that detects Collagen type IV 6 chain (COL4A6). It targets an epitope within the C-terminal region.
      IsotypeIgG2bκ
      Species Reactivity
      • Rat
      Species Reactivity NoteRat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Col4a6
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~45 kDa observed for GST-tagged recombinant fragment; 163.51 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in GST-tagged recombinant COL4A6.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GST-tagged recombinant NC1 domain of Rat COL4A6.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABT1481-25UG 04065270938039