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MABS1259 Anti-CYP51A1 Antibody, clone N6-P2H5*G8

Anti-CYP51A1 Antibody, clone N6-P2H5*G8, Cat. No. MABS1259, is a highly specific mouse monoclonal antibody that targets CYP51A1, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-CYP51A1 Antibody, clone N6-P2H5*G8: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Signaling Gene Symbol: CYP51A1, CYP51 UniProt Number: Q16850
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MABS1259
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1259
      DescriptionAnti-CYP51A1 Antibody, clone N6-P2H5*G8
      Alternate Names
      • Lanosterol 14-alpha demethylase
      • CYP51A1
      • CYPL1
      • CYPLI
      • Cytochrome P450 51A1
      • Cytochrome P450, family 51, subfamily A, polypeptide 1
      • Cytochrome P450-14DM
      • Cytochrome P45014DM
      • Cytochrome P450L1
      • Cytochrome P450LI
      • LDM
      • Sterol 14-alpha demethylase
      Background InformationLanosterol 14-alpha demethylase (EC 1.14.13.70; UniProt Q16850; also known as CYP51A1, CYPL1, CYPLI, Cytochrome P450 51A1, Cytochrome P450, family 51, subfamily A, polypeptide 1, Cytochrome P450-14DM, Cytochrome P45014DM, Cytochrome P450L1, Cytochrome P450LI, LDM, Sterol 14-alpha demethylase) is encoded by the CYP51A1 (also known as CYP51) gene (Gene ID 1595) in human. Cytochromes P450 (CYP) proteins are primarily membrane-associated oxidases located either in the inner membrane of mitochondria or in the endoplasmic reticulum where they function as the terminal enzymes in electron transfer chains. In addition to processing endogenous substrates, CYPs also function to metabolize exogenous drugs and potentially toxic chemicals. The human CYP superfamily consisits of 57 genes and more than 59 pseudogenes divided into 18 families and 43 subfamilies. CYP51A1 represents the only member of the CYP51 subfamily and is involved in the conversion of lanosterol to 4,4-dimethylcholesta-8(9),14,24-trien-3 -ol. Products generated by the CYP51 reaction are vital intermediates in pathways leading to the formation of cholesterol in humans, ergosterol in fungi, and other types of sterols in plants. Sterols play an important structural role in the regulation of membrane fluidity and permeability, which influence the functions of enzymes, ion channels, and other cell components embedded within.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CYP51A1 Antibody, clone N6-P2H5*G8, Cat. No. MABS1259, is a highly specific mouse monoclonal antibody that targets CYP51A1, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: Clone N6-P2H5*G8 hybridoma culture supernatant detected an upregulated CYP51A1 immunoreactivity in human colon carcinoma tissue relative to that in normal liver tissue.

      Immunohistochemistry Analysis: A representative lot detected increased CYP51A1 expression in formalin-fixed, paraffin-embedded primary colorectal cancer tissue than in normal colonic mucosa. Significantly reduced CYP51A1 expression was found in lymph node metastasis compared to Dukes C (stage 3) colorectal cancer (Swan, R., et al. (2016). Oncotarget. In press).

      Western Blotting Analysis: A representative lot detected a target band in lysate from CYP51A1-transfected, but not from untransfected, cells (Swan, R., et al. (2016). Oncotarget. In press).
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponging to a sequence near the C-terminus of human/mouse/rat CYP51A1.
      CloneN6-P2H5*G8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone N6-P2H5*G8 was raised against a CYP51A1 C-terminal sequence 100% conserved among human, mouse, and rat species. Target band was detected only in lysate from CYP51A1-transfected, but not from untransfected, cells (Swan, R., et al. (2016). Oncotarget. In press).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse and Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CYP51A1
      • CYP51
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~50 kDa observed. 56.81/46.31 kDa (human isoform 1/2), 56.78 kDa (mouse), and 56.71 kDa (rat) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human liver microsome lysate.

      Western Blotting Analysis: A 1:125 dilution of this antibody detected CYP51A1 in 50 µg of human liver microsome lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABS1259 04054839034992