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MAB13170 Anti-Cav1.2 calcium channel Antibody, clone L57/46

This Anti-Cav1.2 calcium channel Antibody, clone L57/46 is validated for use in WB, IH for the detection of Cav1.2 calcium channel.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: CACH2, CACN2, TS, CACNL1A1, CaV1.2, CCHL1A1, Cav1.2 UniProt Number: P15381
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MAB13170
100 µg  
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, R, RbWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB13170
      DescriptionAnti-Cav1.2 calcium channel Antibody, clone L57/46
      Alternate Names
      • calcium channel, voltage-dependent, L type, alpha 1C subunit1
      • voltage-gated calcium channel alpha subunit Cav1.2
      • calcium channel, L type, alpha 1 polypeptide, isoform 1, cardic muscle
      • calcium channel, cardic dihydropyridine-sensitive, alpha-1 subunit
      • voltage-gated L-type calcium channel Cav1.2 alpha 1 subunit, splice variant 10*
      • DHPR, alpha-1 subunit
      • Voltage-gated calcium channel subunit alpha Cav1.2
      • Calcium channel, L type, alpha-1 polypeptide, isoform 1, cardiac muscle
      Background InformationThis gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Many alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
      References
      Product Information
      FormatPurified
      Control
      • Mouse brain tissue lysate
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Cav1.2 calcium channel Antibody, clone L57/46 is validated for use in WB, IH for the detection of Cav1.2 calcium channel.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: 1:300 dilution of this antibody has been shown to detect Cav1.2 calium channel in human kidney tissue.
      Biological Information
      ImmunogenRecombinant protein corresponding to rabbit Cav1.2 calcium channel at the cytoplasmic domain proximal to the C-terminus.
      EpitopeCytoplasmic domain
      CloneL57/46
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes Cav1.2 calcium channel at the cytoplasmic domain.
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      • Human
      • Rat
      • Rabbit
      Species Reactivity NoteDemonstrated to react with mouse and human. Predicted to react with rat and rabbit based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CACH2
      • CACN2
      • TS
      • CACNL1A1
      • CaV1.2
      • CCHL1A1
      • Cav1.2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function (By similarity).

      SUBUNIT STRUCTURE: Voltage-dependent calcium channels are multisubunit complexes, consisting of alpha-1, alpha-2, beta and delta subunits in a 1:1:1:1 ratio. The channel activity is directed by the pore-forming and voltage-sensitive alpha-1 subunit. In many cases, this subunit is sufficient to generate voltage-sensitive calcium channel activity. The auxiliary subunits beta and alpha-2/delta linked by a disulfide bridge regulate the channel activity. Interacts with CABP1 and CACNA2D4 By similarity. Interacts (via C-terminal CDB motif) with CABP5; in a calcium-dependent manner (By similarity).

      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. Cell membrane. Note: The interaction between RRAD and CACNB2 regulates its trafficking to the cell membrane. TISSUE SPECIFICTY: Expression in cardiac muscle. In lung, expressed in airway and vascular smooth muscle cells.

      DOMAIN: Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.

      Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel.

      PTM: Phosphorylation by PKA activates the channel.

      SEQUENCE SIMILARITIES: Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. (View classification).
      Molecular Weight Observed at ~ 240 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in mouse brain tissue lysate.

      Western Blot Analysis: 2 µg/mL of this antibody detected Cav1.2 calcium channel on 10 µg of mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MAB13170 04053252518690

      Documentation

      Anti-Cav1.2 calcium channel Antibody, clone L57/46 Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-Cav1.2 calcium channel Antibody, clone L57/46 Certificados de análisis

      CargoNúmero de lote
      Anti-Cav1.2 calcium channel, clone L57/46 - 2127821 2127821
      Anti-Cav1.2 calcium channel, clone L57/46 - 2394046 2394046
      Anti-Cav1.2 calcium channel, -2550884 2550884
      Anti-Cav1.2 calcium channel, -2579911 2579911
      Anti-Cav1.2 calcium channel, -2667314 2667314
      Anti-Cav1.2 calcium channel, -2716676 2716676
      Anti-Cav1.2 calcium channel, -2727221 2727221
      Anti-Cav1.2 calcium channel, -2762311 2762311
      Anti-Cav1.2 calcium channel, -2807063 2807063
      Anti-Cav1.2 calcium channel, clone L57/46 3030288
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