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17-347B Anti-Citrulline (Modified) Detection Kit

The Anti-Citrulline (Modified) Detection Kit provides reagents for the modification and detection of citrulline-containing proteins by Western blot (immublot) analysis.

Anti-Citrulline (Modified) Detection Kit: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology
17-347B
1 kit  Kit capacity: 10 western blots
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      Descripción

      Replacement Information

      Tabla espec. clave

      Species ReactivityKey ApplicationsHost
      AWBHuman Recombinant
      Description
      Catalogue Number17-347B
      Replaces17-347
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Citrulline (Modified) Detection Kit
      OverviewThe Anti-Citrulline (Modified) Detection Kit provides reagents for the modification and detection of citrulline-containing proteins by Western blot analysis. Modification of citrulline residues is created by a chemical reaction with 2,3-butanedione monoxime and antipyrine in a strong acid solution. Detection of the modified citrulline proteins uses a standard immunoblot protocol with an anti-Citrulline (modified) Human monoclonal antibody and a goat anti-Human IgG secondary antibody horseradish peroxidase (HRP) conjugate. This type of citrulline modification ensures the detection of citrulline residues in proteins regardless of neighboring amino acid sequences.
      Materials Required but Not Delivered1. 98% H2SO4 (Sulfuric Acid)
      2. 85% H3PO4 (Phosphoric Acid)
      3. TBS-TWEEN® Tablets (EMD Millipore catalog #524753)
      4. Luminata Crescendo Western HRP substrate (EMD Millipore catalog #WBLUR100)
      5. Non-fat dry milk (EMD Millipore Catalog # 20-200)
      Background InformationCitrullination or deimination is the post-translational modification of arginine residues to citrulline residues in proteins. This process is catalyzed by enzymes called peptidylarginine deiminases (PADs).
      References
      Product Information
      Components
      • Anti-Modified Citrulline, clone C4 (Part number MABS487). One vial containing 100 µL of Protein A purified recombinant human monoclonal IgG1λ in PBS containing 0.05% sodium azide.
      • Goat Anti-Human IgG, HRP Conjugate: (Part number CS216591). One vial containing 50 µL of Goat anti-Human IgG conjugated to horseradish peroxidase (HRP) in PBS, pH 7.6, with 7.5 mg/mL BSA and diluted with Stabilzyme® and glycerol to 50%.
      • Reagent B: (Part number 20-253). One bottle containing 50 mL of 0.5% 2,3-butanedione monoxime, 0.25% antipyrine, 0.5 M acetic acid.
      • 0.5% FeCl3: (Part number 20-255). One bottle containing 4 mL of 0.5% FeCl3. Frozen solution.
      Quality LevelMQ300
      Applications
      ApplicationThe Anti-Citrulline (Modified) Detection Kit provides reagents for the modification and detection of citrulline-containing proteins by Western blot (immublot) analysis.
      Key Applications
      • Western Blotting
      Biological Information
      HostHuman Recombinant
      Species Reactivity
      • All
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore Anti-Modified Citrulline monoclonal antibody at 2-8°C. Store Goat Anti-Human IgG, HRP Conjugate, Reagent B, and 0.5% FeCl3 at -20°C for 4 months from date of receipt.
      Packaging Information
      Material Size1 kit
      Material PackageKit capacity: 10 western blots
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      17-347B 04055977174137

      Documentation

      Anti-Citrulline (Modified) Detection Kit Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-Citrulline (Modified) Detection Kit Certificados de análisis

      CargoNúmero de lote
      Anti-Citrulline (Modified) Detection Kit - 3017763 3017763
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      Anti-Citrulline (Modified) Detection Kit - 3166769 3166769
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      Anti-Citrulline (Modified) Detection Kit - 3314359 3314359
      Anti-Citrulline (Modified) Detection Kit - 3393749 3393749
      Anti-Citrulline (Modified) Detection Kit - 3478349 3478349
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