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CP17 Anti-Collagen Type I Mouse mAb (I-8H5)

Anti-Collagen Type I Mouse mAb (I-8H5): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
CP17
  
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      Descripción

      Replacement Information

      Tabla espec. clave

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes collagen type I at 95 kDa and 210 kDa under reducing conditions. May also cross-react with type II collagen. Does not cross-react with any other collagens.
      Catalogue NumberCP17
      Brand Family Calbiochem®
      Application Data
      Detection of human collagen type 1 by immunoblotting. Sample: Tissue from human placental. Primary antibody: Anti-Collagen Type I Mouse mAb (I-8H5) (Cat. No. CP17) (4 µg/ml). Detection: chemiluminescence.
      References
      ReferencesKarsenty, G., et al. 1991. J. Biol. Chem. 266, 24842.
      Mayne, R. and Burgeson, R.E. 1987. Structure and Function of Collagen Types, Academic Press, Orlando, FL.
      Weiner, F.R., et al. 1987. J. Biol. Chem. 262, 6955.
      Kreig, T., et al. 1985. Ann. N.Y. Acad. Sci. 460, 375.
      Laurent, G.J. 1985. Ciba Found. Symp. 114, 227.
      de Wet, W.J., et al. 1983. J. Biol. Chem. 258, 7721.
      Borsch, A.S. and Byers, P. 1981. Proc. Natl. Acad. Sci. USA 78, 5141.
      Product Information
      DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
      FormLiquid
      FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
      Positive controlPurified collagen type I
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesFrozen Sections Van der Loos, C.M. et al. 1994. Histochem. J. 26, 347. Paraffin Sections Kumagai, J., et al. 1994. J. Anat. 185, 279.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesFrozen Sections (1-10 µg/ml; see application references)
      Immunoblotting (5 µg/ml)
      Paraffin Sections (1-10 µg/ml; trypsin pre-treatment required; see application references)
      Application CommentsIt may also react with Type II collagen. It does not react with other collagen classes. In immunoblotting bands of ~95 kDa and ~210 kDa are obtained. Higher molecular bands corresponding to aggregates are also seen. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified, human collagen type I
      ImmunogenHuman
      CloneI-8H5
      HostMouse
      IsotypeIgG2a
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      CP17 0

      Documentation

      Referencias bibliográficas

      Visión general referencias
      Karsenty, G., et al. 1991. J. Biol. Chem. 266, 24842.
      Mayne, R. and Burgeson, R.E. 1987. Structure and Function of Collagen Types, Academic Press, Orlando, FL.
      Weiner, F.R., et al. 1987. J. Biol. Chem. 262, 6955.
      Kreig, T., et al. 1985. Ann. N.Y. Acad. Sci. 460, 375.
      Laurent, G.J. 1985. Ciba Found. Symp. 114, 227.
      de Wet, W.J., et al. 1983. J. Biol. Chem. 258, 7721.
      Borsch, A.S. and Byers, P. 1981. Proc. Natl. Acad. Sci. USA 78, 5141.
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision06-July-2007
      ApplicationFrozen Sections (1-10 µg/ml; see application references)
      Immunoblotting (5 µg/ml)
      Paraffin Sections (1-10 µg/ml; trypsin pre-treatment required; see application references)
      Application Data
      Detection of human collagen type 1 by immunoblotting. Sample: Tissue from human placental. Primary antibody: Anti-Collagen Type I Mouse mAb (I-8H5) (Cat. No. CP17) (4 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes ~95 kDa and ~210 kDa bands representing type I collagen protein (see comments).
      BackgroundCollagens are the major fibrous structural elements of cartilage, tendon, skin, bone, lung, and blood vessels. Their function is to hold cells together and thereby form discrete tissues. Collagens form insoluble fibers that have a high intrinsic tensile strength. Structurally, the collagens are composed of three polypeptide chains, α chains, which exist as three distinct subclasses, each of which in turn is made up of numerous subtypes. Type I collagen, is the most abundant fibrillar collagen and is preferentially synthesized in bone, skin, and tendon. It is composed of two α1(I) chains and one α2(I) chain in a 2:1 stoichiometry. The synthesis of type I collagen is modified by a variety of factors including growth factors, hormones, and mutated oncogenes. Increase in synthesis is seen in fibrosis conditions including that occuring in lung, liver, and scleroderma.
      HostMouse
      Immunogen speciesHuman
      Immunogenpurified, human collagen type I
      CloneI-8H5
      IsotypeIgG2a
      Specieshuman, rabbit
      Positive controlPurified collagen type I
      FormLiquid
      FormulationIn 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsIt may also react with Type II collagen. It does not react with other collagen classes. In immunoblotting bands of ~95 kDa and ~210 kDa are obtained. Higher molecular bands corresponding to aggregates are also seen. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesKarsenty, G., et al. 1991. J. Biol. Chem. 266, 24842.
      Mayne, R. and Burgeson, R.E. 1987. Structure and Function of Collagen Types, Academic Press, Orlando, FL.
      Weiner, F.R., et al. 1987. J. Biol. Chem. 262, 6955.
      Kreig, T., et al. 1985. Ann. N.Y. Acad. Sci. 460, 375.
      Laurent, G.J. 1985. Ciba Found. Symp. 114, 227.
      de Wet, W.J., et al. 1983. J. Biol. Chem. 258, 7721.
      Borsch, A.S. and Byers, P. 1981. Proc. Natl. Acad. Sci. USA 78, 5141.
      Application referencesFrozen Sections Van der Loos, C.M. et al. 1994. Histochem. J. 26, 347. Paraffin Sections Kumagai, J., et al. 1994. J. Anat. 185, 279.