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MABT1475-100UG Anti-Cytokeratin-8 Antibody, clone 1E8

Anti-Cytokeratin-8, clone 1E8, Cat. No. MABT1475, is a mouse monoclonal antibody that detects Cytokeratin-8 and is tested for use in Western Blotting, Inhibition, Immunocytochemistry, and Immunohistochemistry,.

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100 μg  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberMABT1475-100UG
      DescriptionAnti-Cytokeratin-8 Antibody, clone 1E8
      Alternate Names
      • Keratin
      • type II cytoskeletal 8
      • Keratin-8 (K8)
      • Type-II keratin Kb8
      • KRT8
      • CYK8
      Background InformationKeratin, type II cytoskeletal 8 (UniProt: P05787; also known as Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8) is encoded by the KRT8 (also known as CYK8) gene (Gene ID: 3856) in human. Cytokeratins belong to a diverse group of intermediate filaments that participate in differentiation and maintain the structural integrity of epithelial cells. They share a common structural organization: a central -helical rod domain flanked by head and tail domains. An important unique feature of cytokeratins is their epithelial cell-type-specific expression. CK8 is expressed in abundance in the epithelia of colon, bladder, ileum, and stomach. It is a type II, neutral to basic protein that together with cytokeratin-19 (KRT19) helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK-8 can undergo phosphorylation on three major serine residues: Serine 23, 431, and 73. Serine 23 is highly conserved in all type II keratins. Phosphorylation at serine 73 is reported to increase during cellular stress. However, under normal conditions serine 73 remains largely dephosphorylated. It can also undergo O-glycosylation in a cell cycle-dependent manner and glycosylation increases its solubility and reduces stability by inducing proteasomal degradation. CK8 expression has been correlated with malignancy in leukoplakia and carcinomas of the head and neck and its expression has been observed in all non-small-cell lung cancers.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in PBS without azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-Cytokeratin-8, clone 1E8, Cat. No. MABT1475, is a mouse monoclonal antibody that detects Cytokeratin-8 and is tested for use in Western Blotting, Inhibition, Immunocytochemistry, and Immunohistochemistry,.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Inhibition
      • Immunocytochemistry
      Application NotesTested Applications

      Immunohistochemistry Applications: A representative lot detected Cytokeratin-8 in Immunohistochemistry application (Sfakianos, J.P., et al. (2020). Nat Commun. 11(1):2540).

      Western Blotting Analysis: A representative lot detected Cytokeratin-8 in Western Blotting application (Hembrough, T.A., et al. (1996). J Biol Chem. 271(41):25684-91).

      Immunocytochemistry Analysis: A representative lot detected Cytokeratin-8 in Immunocytochemistry application (Hembrough, T.A., et al. (1996). J Biol Chem. 271(41):25684-91).

      Inhibition Assay: An Anti-Cytokeratin-8, clone 1E8 Fab decreased plasminogen binding to breast cancer cell lines in Inhibition Assay (Hembrough, T.A., et al. (1996). J Biol Chem. 271(41):25684-91).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal of human Cytokeratin-8.
      EpitopeC-terminal
      Clone1E8
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 1E8 is a mouse monoclonal antibody that detects human Cytokeratin-8. It targets an epitope within 12 amino acids from the C-terminal part.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Canine, Monkey based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KRT8
      Purification MethodProtein G purified
      UniProt Number
      Molecular WeightTarget molecular weight ~54 kDa observed. 53.70 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HaCaT cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Cytokeratin-8 in HaCaT cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABT1475-100UG 04065272290296

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