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NE1038 Anti-F13A1 Mouse mAb (M1)

Anti-F13A1 Mouse mAb (M1): Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: Anti-Coagulation Factor13A1

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      Descripción

      Replacement Information

      Tabla espec. clave

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the F13A1 protein.
      Catalogue NumberNE1038
      Brand Family Calbiochem®
      SynonymsAnti-Coagulation Factor13A1
      Application Data
      Detection of human F13A1 by immunocytochemistry using an in situ proximity ligation assay. Sample: Huh7 cells. Primary antibody: Anti-F13A1 Mouse mAb (M1) (Cat. No. NE1038) (1:50) and an Anti-HSPB1 rabbit pAb (1:1200). Detection: fluorescence using Duolink® Detection kit U13 (red) with DAPI counterstain.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunocytochemistry (1:50)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant, human F13A1 (aa 1-733), expressed as a GST fusion protein
      CloneM1
      HostMouse
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      NE1038 0

      Documentation

      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-November-2010 RFH
      SynonymsAnti-Coagulation Factor13A1
      ApplicationELISA (see comments)
      Immunocytochemistry (1:50)
      Application Data
      Detection of human F13A1 by immunocytochemistry using an in situ proximity ligation assay. Sample: Huh7 cells. Primary antibody: Anti-F13A1 Mouse mAb (M1) (Cat. No. NE1038) (1:50) and an Anti-HSPB1 rabbit pAb (1:1200). Detection: fluorescence using Duolink® Detection kit U13 (red) with DAPI counterstain.
      DescriptionPurified mouse monoclonal antibody. Recognizes the F13A1 protein.
      BackgroundCoagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Plasma factor XIII is a heterotetramer composed of 2 A subunits and 2 B subunits. The A subunits have catalytic function, and the B subunits do not have enzymatic activity and may serve as plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIIIa, as platelet factor XIII. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits; and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion.
      HostMouse
      ImmunogenFull-length, recombinant, human F13A1 (aa 1-733), expressed as a GST fusion protein
      CloneM1
      IsotypeIgG
      Specieshuman
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review