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07-1500 Anti-G Protein Giα 1/2 Antibody

Anti-G Protein Giα 1/2 Antibody is an antibody against G Protein Giα 1/2 for use in WB, IH(P).

Anti-G Protein Giα 1/2 Antibody: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Signaling Research Sub-Category: GPCR, cAMP/cGMP & Calcium Signaling Gene Symbol: GNAI1, Gi UniProt Number: P63096
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07-1500
100 µg  
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      Descripción

      Replacement Information
      Replacement Information07-1500 is a recommended replacement for AB4067

      Ofertas especiales

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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, H, BWB, IH(P)RbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-1500
      ReplacesAB4067
      DescriptionAnti-G Protein Giα 1/2 Antibody
      Background InformationGTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Gi belongs to the pertussis toxin sensitive G-proteins. Gi consists of three different subtypes of alpha-subunits (alphai1, alphai2 and alphai3).
      References
      Product Information
      FormatPurified
      Control
      • L6 Cell Lysate
      PresentationPurified rabbit polyclonal IgG in phosphate buffered saline with 0.08% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-G Protein Giα 1/2 Antibody is an antibody against G Protein Giα 1/2 for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry(paraffin):
      Representative testing from a previous lot.
      Optimal Staining of GI ALPHA 1/2 With Citrate pH 6.0 Epitope Retrieval: Rat Cerebellum GI ALPHA 1/2 representative staining pattern/morphology in rat cerebellum. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPeptide corresponding to amino acids 345-354 of Gi1 and 346-355 of Gi2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes G protein subunit αi1.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Rat
      • Human
      • Bovine
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryGuanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain (GNAO1; MIM 139311); and transducin-1 (GNAT1; MIM 139330) and transducin-2 (GNAT2; MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218]; Bray et al., 1987 [PubMed 3110783]). Suki et al. (1987) [PubMed 2440724] concluded that the human genome contains at least 3 nonallelic genes for alpha-i-type subunits of G protein; see, e.g, GNAI2 (MIM 139360), GNAI3 (MIM 139370), and GNAIH (MIM 139180).[supplied by OMIM] ...
      Gene Symbol
      • GNAI1
      • Gi
      Purification MethodPurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P63096 # Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(i) proteins are involved in hormonal regulation of adenylate cyclase: they inhibit the cyclase in response to beta-adrenergic stimuli.
      SIZE: 354 amino acids; 40361 Da
      SUBUNIT: G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with GPSM1 (By similarity).
      SIMILARITY: SwissProt: P63096 ## Belongs to the G-alpha family. G(i/o/t/z) subfamily.
      Molecular Weight~40 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on L6 lysates.

      Western Blot Analysis: 1:500 dilution of this lot detected G protein Gialpha 1/2 on 10 μg of L6 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable at -20°C in undiluted aliquots for up to 12 months from date of receipt.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      07-1500 04053252366048

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      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies