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ABE2590-25UL Anti-H3K4me3Q5dopaminyl

Anti-H3K4me3Q5dopaminyl, Cat. No. ABE2590, is a rabbit polyclonal antibody that detects Histone H3.1 dopaminyated on glutamine 5 and is tested for use in Dot Blot, Peptide Inhibition Assay, and Western Blotting.

ABE2590-25UL
25 µL  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberABE2590-25UL
      DescriptionAnti-H3K4me3Q5dopaminyl
      Alternate Names
      • Dopaminylated Histone H3
      • H3Q5dopaminylated
      Background InformationHistone H3.1t (UniProt: Q16695; also known as H3/t, H3t, H3/g) is encoded by the HIST3H3 (also known as H3FT) gene (Gene ID: 8290) in human. Histone H3 has two main variants, H3.1 and H3.3, which show different genomic localization patterns in animals. The H3.1 and H3.3 complexes also possess distinct histone chaperones, CAF-1 and HIRA, which play important role in mediating DNA-synthesis-dependent and -independent nucleosome assembly. Histone H 3.1 is a core component of nucleosome that is present only in mammals. It is expressed during S phase, then its expression decreases significantly as cell division slows down during the process of differentiation. Histone H 3.1 expression is shown to be replication dependent. It serves as the canonical histone, which is incorporated during DNA replication.
      Histones are dynamic proteins that undergo multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Histone 3.1 is also shown to undergo serotonylation of glutamine at position 5 (Q5ser) in organisms that produce serotonin. Recently, it has been reported that Histone H3 can undergo dopaminylation ((H3Q5dop), which can potentiate the binding of adjacent histone post-translational modification interacting proteins and play a vital role in dopaminergic neuronal transcription. This rabbit polyclonal antibody specifically binds to Histone H3 dopaminylated on glutamine 5.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-H3K4me3Q5dopaminyl, Cat. No. ABE2590, is a rabbit polyclonal antibody that detects Histone H3.1 dopaminyated on glutamine 5 and is tested for use in Dot Blot, Peptide Inhibition Assay, and Western Blotting.
      Key Applications
      • Dot Blot
      • Peptide Inhibition Assay
      • Western Blotting
      Application NotesDot Blot Analysis: A 1:1,000 dilution from a representative lot detected H3K4me3Q5dopaminyl in H3(K4me3Q5dopaminyl) & H3 control peptide (Courtesy of Ian Maze Lab, Icahn School of Medicine at Mount Sinai, New York).

      Western Blotting Analysis: A 1:200 dilution from a representative lot detected H3K4me3Q5dopaminyl in human and rat ventral tegmental area (Courtesy of Ian Maze Lab, Icahn School of Medicine at Mount Sinai, New York).

      Peptide Inhibition Assay: Target band detection in H3K4me3Q5dopaminyl was prevented by preblocking of a representative lot with the immunogen peptide, but not with a control peptide.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 10 amino acids from the N-terminal region of human Histone H3 with trimethylated lysine 4 and dopaminylated on glutamine 5.
      EpitopeN-terminus
      Concentration0.03 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Histone H3 dopaminylated on glutamine 5.
      IsotypeIgG
      Species Reactivity
      • Rat
      • Human
      Species Reactivity NoteHuman, Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST3H3
      • H3FT
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight15.51 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Dot Blot in H3(K4me3Q5dopaminyl) & H3 control peptide.



      Western Blotting Analysis: A 1:500 dilution of this antibody detected H3K4me3Q5dopaminyl, but not the H3 control peptide.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      ABE2590-25UL 04061842196333