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AP1161 Anti-IL20 Mouse mAb (2H8)

AP1161
  
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      Descripción

      Replacement Information

      Tabla espec. clave

      Host
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      Description
      Overview

      This product has been discontinued.





      Recognizes the ~22 kDa IL20 protein in A431 and K562 cells.
      Catalogue NumberAP1161
      Brand Family Calbiochem®
      SynonymsAnti-Interleukin 20
      Application Data
      Detection of human IL20 by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-IL20 Mouse mAb (2H8) (Cat. No. AP1161) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human IL20 by immunoblotting. Sample: Whole cell lysate from K562 cells. Primary antibody: Anti-IL20 Mouse mAb (2H8) (Cat. No. AP1161) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlA431 cells, K562 cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 67-177 of human IL20, expressed as a GST fusion protein
      Clone2H8
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      AP1161 0

      Documentation

      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-October-2010 RFH
      SynonymsAnti-Interleukin 20
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human IL20 by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-IL20 Mouse mAb (2H8) (Cat. No. AP1161) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human IL20 by immunoblotting. Sample: Whole cell lysate from K562 cells. Primary antibody: Anti-IL20 Mouse mAb (2H8) (Cat. No. AP1161) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~22 kDa IL20 protein.
      BackgroundIL20 is a cytokine structurally related to interleukin 10 (IL10). This cytokine has been shown to transduce its signal through the signal transducer and activator of transcription 3 (STAT3) in keratinocytes.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 67-177 of human IL20, expressed as a GST fusion protein
      Clone2H8
      IsotypeIgG₁
      Specieshuman
      Positive controlA431 cells, K562 cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review