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MABF3137-25UG Anti-MXRA8 Antibody, clone 9E9

Anti-MXRA8, clone 9E9 , Cat. No. MABF3137, is a mouse monoclonal antibody that detects MXRA8 and is tested for use in Flow Cytometry, Inhibition Assay, and Western Blotting.

MABF3137-25UG
25 μg  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberMABF3137-25UG
      DescriptionAnti-MXRA8 Antibody, clone 9E9
      Alternate Names
      • Matrix remodeling-associated protein 8
      • Dual Ig domain-containing cell adhesion molecule
      • Limitrin
      Background InformationMatrix remodeling-associated protein 8 (UniProt: Q9BRK3; also known as Dual Ig domain-containing cell adhesion molecule, DICAM, MXRA8, Limitrin) is encoded by the MXRA8 gene (Gene ID: 54587) in human. MXRA8/DICAM is a single-pass type I, homodimeric, membrane glycoprotein that is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 20-341), a transmembrane domain (aa 342-362), and a cytoplasmic domain (aa 363-442). It is highly expressed at the areas of cell-cell contacts. It is also detected in the soluble fraction of the nucleus. Its high expression is observed in the brain where it localizes to glia limitans formed by the end feet of astrocytes surrounding capillaries, and beneath the pia mater. It is also expressed in intercalated disks in the heart and has been detected in pancreatic -cells. It modulates the activity of various signaling pathways via binding to integrin V:β3 via its RGD motif (aa 252-254). It plays a role in cartilage formation where it promotes proliferation and maturation of growth plate chondrocytes and stimulates the formation of primary cilia in chondrocytes. MXRA8 also plays a role in the maturation and maintenance of the blood-brain barrier. It is reported to function as a cell trafficking molecule that is preferentially expressed by T helper 17 (TH17)-polarized CD4+ T lymphocytes. Its expression on circulating CD4+ T cells is increased in patients with active relapsing-remitting multiple sclerosis (RRMS) and in progressive forms of multiple sclerosis. Expression of its ligands also increases on the blood-brain barrier endothelium upon inflammation and in multiple sclerosis lesions. Four isoforms of MXRA8 have been described that are produced by alternative splicing. (Ref.: Charabati, M., et al. (2022). Sci. Transl. Med. 14; eabj0473; Zhang, R., et al. (2018). Nature. 557(7706); 570-574).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
      Quality LevelMQ200
      Applications
      ApplicationAnti-MXRA8, clone 9E9 , Cat. No. MABF3137, is a mouse monoclonal antibody that detects MXRA8 and is tested for use in Flow Cytometry, Inhibition Assay, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Western Blotting
      • Inhibition
      Application NotesTested Applications

      Inhibition Assay: A representative lot block MXRA8 activity and reduced TH17 cell trafficking across the blood brain barrier. (Charabati, M., et al. (2022). Sci Transl Med. 14(626): eabj0473).

      Flow Cytometry Analysis: A representative lot detected MXRA8 in Flow Cytometry applications (Charabati, M., et al. (2022). Sci Transl Med.14(626): eabj0473).

      Western Blotting Analysis: A representative lot detected MXRA8 in Western Blotting applications (Charabati, M., et al. (2022). Sci Transl Med. 14(626): eabj0473).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the extracellular domain within the N-terminal region of human Matrix remodeling-associated protein 8 (MXRA8).
      EpitopeN-terminal
      Clone9E9
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 9E9 is a mouse monoclonal antibody that detects Matrix remodeling-associated protein 8 (MXRA8). It targets an epitope within 15 amino acids from the N-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MXRA8
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~49 kDa observed. 49.13 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in THP-1 cells.

      Flow Cytometry Analysis: 1.0 µg of this antibody detected MXRA8 in one million THP-1 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABF3137-25UG 04065270321848

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