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475948 Anti-Munc-18 (58-70) Rabbit pAb

Anti-Munc-18 (58-70) Rabbit pAb: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
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      Descripción

      Replacement Information

      Tabla espec. clave

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~67 kDa Munc-18 protein.
      Catalogue Number475948
      Brand Family Calbiochem®
      Application Data
      Detection of rat Munc-18 by immunoprecipitation. Samples: Whole tissue extract from rat brain left untreated (lane 1) and 750 µg total protein from above extract immunoprecipitated with 1 µg, (lane 2) 5 µg, (lane 3) and 10 µg (lane 4). Primary antibody: Anti-Munc-18 (58-70) Rabbit pAb (Cat. No. 475948) (assay dependent of Promary Antibody). Detection: chemiluminescence.
      References
      ReferencesKalidas, S., et al. 2000. Acta. Neuropathol. 99, 191.
      Takahashi, M., et al. 2000. J. Neurol. Sci. 172, 63.
      Product Information
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1 µg/ml)
      Immunoprecipitation (see comments)
      Application CommentsThis antibody is also reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogena synthetic peptide (VEDINKRREPIPS) corresponding to amino acids 58-70 of human Munc-18-2 isoform
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      475948 0

      Documentation

      Anti-Munc-18 (58-70) Rabbit pAb Certificados de análisis

      CargoNúmero de lote
      475948

      Referencias bibliográficas

      Visión general referencias
      Kalidas, S., et al. 2000. Acta. Neuropathol. 99, 191.
      Takahashi, M., et al. 2000. J. Neurol. Sci. 172, 63.
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-August-2007 RFH
      ApplicationImmunoblotting (1 µg/ml)
      Immunoprecipitation (see comments)
      Application Data
      Detection of rat Munc-18 by immunoprecipitation. Samples: Whole tissue extract from rat brain left untreated (lane 1) and 750 µg total protein from above extract immunoprecipitated with 1 µg, (lane 2) 5 µg, (lane 3) and 10 µg (lane 4). Primary antibody: Anti-Munc-18 (58-70) Rabbit pAb (Cat. No. 475948) (assay dependent of Promary Antibody). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~67 kDa Munc-18 protein.
      BackgroundMunc-18 is a neuron-specific protein of ~67 kDa and is known for its ability to bind with syntaxin and also to copurify with neuronal Cdc2-like kinase. Munc-18 plays a key role in synaptic vesicle fusion events with the plasma membrane. Due to its ability to be expressed in postmitotic neurons of adult human brain and in tumors of neuronal origin, it has been suggested that Munc-18 may be an important molecular tool in understanding the growth and differentiation of the nervous system in general. Two Munc-18 isoforms have been identified. Munc-18-1 is primarily expressed in brain, while Munc-18-2 is expressed in most tissues.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (VEDINKRREPIPS) corresponding to amino acids 58-70 of human Munc-18-2 isoform
      IsotypeIgG
      Specieshuman, rat
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody is also reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the optimal working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesKalidas, S., et al. 2000. Acta. Neuropathol. 99, 191.
      Takahashi, M., et al. 2000. J. Neurol. Sci. 172, 63.