Millipore Sigma Vibrant Logo

07-1799 Anti-NCoA-2 Antibody

View Products on
100 µg  
Recuperando precio...
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Maximum Quantity is
Al finalizar el pedido Más información
Ahorró ()
Solicitar precio
Disponibilidad a confirmar
Disponibilidad a confirmar
Ingrese cantidad 
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

      Ofertas especiales


      Póngase en contacto con el Servicio de Atención al Cliente


      Replacement Information

      Ofertas especiales

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ChIPRbPurifiedPolyclonal Antibody
      Catalogue Number07-1799
      DescriptionAnti-NCoA-2 Antibody
      Alternate Names
      • Transcriptional intermediary factor 2
      • glucocorticoid receptor-interacting protein-1
      • nuclear receptor coactivator 2
      • glucocorticoid receptor interacting protein 1
      • steroid receptor coactivator-2
      • transcriptional mediators/intermediary factor 2
      Background InformationNCoA2 (nuclear receptor coactivator 2) is a transcriptional coactivator for steroid receptors and nuclear receptors. Also known as glucocorticoid receptor interacting protein 1 (GRIP1), steroid receptor coactivator-2 (SRC-2), or transcriptional mediators/intermediary factor 2 (TIF2), this coregulatory protein contains several nuclear receptor interacting domains and an intrinsic histone acetyltransferase activity. NCoA-2 assists nuclear receptors in the upregulation of DNA expression. In cells ligand activated nuclear receptors recruit NCoA-2 to DNA where it acylates histones to make targeted regions accessible to transcriptional machinery.

      Product Information
      • U2 OS cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
      Quality LevelMQ100
      ApplicationUse Anti-NCoA-2 Antibody (rabbit polyclonal antibody) validated in WB to detect NCoA-2 also known as Transcriptional intermediary factor 2, glucocorticoid receptor-interacting protein-1, nuclear receptor coactivator 2.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      A representative lot of this antibody was used for chIP. See Monroe, D.G., et al. (2006) J. Biol. Chem. 281(39):28596-28604.
      Biological Information
      ImmunogenLinear peptide corresponding to human NCoA-2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis antibody recognizes NCoA-2.
      Species Reactivity
      • Human
      Species Reactivity NoteDemonstrated to react with human. Homology to mouse and rat is 95%.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NCOA2
      • GRIP1
      • KAT13C
      • NCoA-2
      • SRC2
      • TIF2
      • bHLHe75
      • hTIF2
      • SRC-2
      • TIF-2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Transcriptional coactivator for steroid receptors and nuclear receptors. Coactivator of the steroid binding domain (AF-2) but not of the modulating N-terminal domain (AF-1). Required with NCOA1 to control energy balance between white and brown adipose tissues.

      SUBUNIT STRUCTURE: Present in a complex containing CARM1 and EP300/P300, and interacts with CARM1 and NR3C2 By similarity. Present in a complex containing NCOA3, IKKA, IKKB, IKBKG and CREBBP. Interacts (via C-terminus) with CREBBP. Interacts with HIF1A, NCOA1, APEX and NR3C1. Interacts with CASP8AP2 and TTLL5/STAMP. Interacts with ESR1, RARA and RXRA.


      DOMAIN: Contains four Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. The LXXLL motifs are essential for the association with nuclear receptors and are, at least in part, functionally redundant. Ref.4

      The LLXXLXXXL motif is involved in transcriptional coactivation and CREBBP/CBP binding.

      Contains 2 C-terminal transcription activation domains (AD1 and AD2) that can function independently.

      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.

      INVOLVEMENT IN DISEASE: Chromosomal aberrations involving NCOA2 may be a cause of acute myeloid leukemias. Inversion inv8(p11;q13) generates the MYST3-NCOA2 oncogene, which consists of the N-terminus part of MYST3/MOZ and the C-terminus part of NCOA2/TIF2. MYST3-NCOA2 binds to CREBBP and disrupts its function in transcription activation.

      SEQUENCE SIMILARITIES: Belongs to the SRC/p160 nuclear receptor coactivator family.

      Contains 1 basic helix-loop-helix (bHLH) domain.

      Contains 1 PAS (PER-ARNT-SIM) domain.
      Molecular Weight~ 160 kDa
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in U2 OS cell lysate.

      Western Blot Analysis: 0.5 µg/ml of this antibody detected SRC2 on 10 µg of U2 OS cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Número de referencia GTIN
      07-1799 04053252401763