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MABF3169-25UG Anti-P-selectin/CD62P Antibody, clone WAPS12.2

Anti-P-selectin/CD62P, clone WAPS12.2, Cat. No. MABF3169, is a mouse monoclonal antibody that detects P-selectin/CD62P and is tested for use in Flow cytometry, Immunohistochemistry (Paraffin), and Inhibition Assay.

MABF3169-25UG
25 μg  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberMABF3169-25UG
      DescriptionAnti-P-selectin/CD62P Antibody, clone WAPS12.2
      Alternate Names
      • CD62 antigen-like family member P
      • Granule membrane protein 140
      • GMP-140
      • Leukocyte-endothelial cell adhesion molecule 3
      • LECAM3
      • Platelet activation dependent granule-external membrane protein
      • PADGEM
      • CD62P
      Background InformationP-selectin (UniProt: P16109; also known as CD62 antigen-like family member P, Granule membrane protein 140, GMP-140, Leukocyte-endothelial cell adhesion molecule 3, LECAM3, Platelet activation dependent granule-external membrane protein, PADGEM, CD62P) is encoded by the SELP (also known as GMRP, GRMP) gene (Gene ID: 6403) in human. CD62P is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-41), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 42-771), a transmembrane domain (aa 772-795), and a cytoplasmic domain (aa 796-830). It serves as a calcium-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. The ligand recognized by CD62P is reported to be sialyl-Lewis X. CD62P mediates the interaction of activated endothelial cells or platelets with leukocytes and facilitates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with selectin P ligand (CD162). Within a few minutes following stimulation with thrombin, histamine, or phorbol 12-myristate 13-acetate (PMA), CD62P is translocated to the cell surface. (Ref.: Somers, WS., et al. (2000). Cell. 103(3); 467-479; Jutila, MA., et al. (1994). J. Immunol. 153(9); 3917-3928).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
      Quality LevelMQ200
      Applications
      ApplicationAnti-P-selectin/CD62P, clone WAPS12.2, Cat. No. MABF3169, is a mouse monoclonal antibody that detects P-selectin/CD62P and is tested for use in Flow cytometry, Immunohistochemistry (Paraffin), and Inhibition Assay.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Flow Cytometry
      • Inhibits Activity/Function
      Application NotesTested Applications

      Inhibition Assay: A representative lot Inhibited the rolling of / T cells on thrombin-activated platelets. (Jutila, M.A., et al. (1994). J Immunol. 153(9):3917-28).

      Flow Cytometry Analysis: A representative lot detected P-selectin/CD62P in Flow Cytometry applications (Jutila, M.A., et al. (1994). J Immunol.153(9):3917-28).

      Immunohistochemistry Applications: A representative lot detected P-selectin (CD62P) in Immunohistochemistry applications (Auvinen, K., et al. (2014). Immunology. 143(3):406-15).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenL1/2m stably transfected with a human P-selectin cDNA construct.
      EpitopeUnknown
      CloneWAPS12.2
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone WAPS12.2 is a mouse monoclonal antibody that detects P-selectin/CD62P.
      IsotypeIgG1
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SELP
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight90.83 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in Human bone marrow tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:25 dilution of this antibody detected P-selectin/CD62P in Human bone marrow tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABF3169-25UG 04065270845252