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ABC1451-100UG Anti-PDGF-D

Anti-PDGF-D, Cat. No. ABC1451, is a rabbit polyclonal antibody that detects Platelet-derived growth factor D in human cells and has been tested for use in Western Blotting.

Anti-PDGF-D: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Apoptosis & Cancer Gene Symbol: PDGFD, IEGF, SCDGFB, MSTP036 UniProt Number: Q9GZP0
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ABC1451-100UG
100 μg  
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      Descripción

      Replacement Information

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC1451-100UG
      DescriptionAnti-PDGF-D
      Alternate Names
      • Platelet-derived growth factor D
      • Iris-expressed growth factor
      • Spinal cord-derived growth factor B
      • SCDGF-B
      Background InformationPlatelet-derived growth factor D (UniProt: Q9GZP0; also known as PDGF-D, Iris-expressed growth factor, Spinal cord-derived growth factor B, SCDGF-B) is encoded by the PDGFD (also known as IEGF, SCDGFB, MSTP036, UNQ1899/PRO4345) gene (Gene ID: 80310) in human. Four PDGF ligands known as PDGF-A, PDGF-B, PDGF-C, and PDGF-D have been described that exert their effect by binding to their cognate receptors. PDGF-D is expressed at high levels in the heart, pancreas, adrenal gland and ovary and at low levels in placenta, liver, kidney, prostate, testis, small intestine, spleen, and colon. PDGF-D is synthesized with a signal peptide (aa 1-18), which is subsequently removed. The latent form of PDGF-D contains a CUB domain at its N-terminal region, a growth factor domain and a hinge region in between. The latent form is activated by the proteolytic action of matriptase that removes the CUB domain, which results in unmasking of the receptor-binding epitopes on the core domain. YR247GR249S within the PDGF-D hinge region is shown to be the cleavage site for removal of the inhibitory CUB domain. PDGF-D plays an important role in wound healing and induces macrophage recruitment, increased interstitial pressure, and blood vessel maturation during angiogenesis. PDGF-D also induces cellular transformation and promotes tumor growth by accelerating the proliferation rate of tumor cells and by stimulation of tumor neovascularization. (Ref.: Huang, W., and Kim, H-R, C (2015). J. Biol. Chem. 290(14); 9162-9170).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS (pH 7.4) with 0.09% sodium azide.
      Applications
      ApplicationAnti-PDGF-D, Cat. No. ABC1451, is a rabbit polyclonal antibody that detects Platelet-derived growth factor D in human cells and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected PDGF-D in human recombinant-PDGF D (Huang, W., et. al. (2015). J Biol Chem. 290(14):9162-70) and LNCaP cells expressing PDGF-D, BS-C-1 cells transfected with the plasmid pTF7-PDGF D:HIS, recombinant PDGF-D (Ustach, C.V., et. al. (2004). Cancer Res. 64(5):1722-9).

      Western Blotting Analysis: A representative lot detected PDGF-D in PBS exchanged condition media collected from LNCaP cells transfected with PDGF-D expression vector (Courtesy of Hyeong-Reh Choi Kim lab at Wayne State University).
      Biological Information
      ImmunogenA linear peptide corresponding to 18 amino acids from the C-terminal half of human Platelet-derived growth factor D.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Platelet-derived growth factor D in human cells. It targets an epitope within the growth factor domain from the C-terminal half.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Rabbit based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PDGFD
      • IEGF
      • SCDGFB
      • MSTP036
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~90 kDa Full Length PDGF D dimer, ~52 kDa PDGF D hemi-dimer,~32 kDa growth factor dimer in the non-reducing conditions; 42.85 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting condition media collected from LNCaP cells transfected with PDGF-D expression vector.

      Western Blotting Analysis: 2 µg/mL of this antibody detected PDGF-D in PBS exchanged condition media collected from LNCaP cells transfected with PDGF-D expression vector.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      ABC1451-100UG 04054839460081