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05-321MG Anti-Phosphotyrosine Antibody, clone 4G10®

Anti-Phosphotyrosine Antibody, clone 4G10 detects level of Phosphotyrosine & has been published & validated for use in IP & WB.

Anti-Phosphotyrosine Antibody, clone 4G10®: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Signaling Research Sub-Category: General Post-translation Modification
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05-321MG
1 mg  
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AIP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-321MG
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phosphotyrosine Antibody, clone 4G10®
      Background InformationSome of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity.
      The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
      References
      Product Information
      FormatPurified
      Control
      • Untreated A-431 (negative control) and EGF treated A-431 (positive control) whole cell lysates.
        Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20°C. The lysate is stable for 6 months at -20°C. Before use, add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
      PresentationPurified mouse monoclonal IgG2bκ in buffer containing PBS, pH 7.4. Liquid at 2-8ºC.
      IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10® (FOX-NY [NS-1 derivative] myeloma x spleen cells).
      Quality LevelMQ100
      Applications
      ApplicationAnti-Phosphotyrosine Antibody, clone 4G10 detects level of Phosphotyrosine & has been published & validated for use in IP & WB.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation:
      2-4 μg of a previous lot immunoprecipitated quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10 cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add: 0.2 mM sodium orthovanadate to the lysis buffer.
      Biological Information
      ImmunogenPhosphotyramine-KLH
      Clone4G10®
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes tyrosine-phosphorylated proteins from all species.
      IsotypeIgG2bκ
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Modifications
      • Phosphorylation
      Purification MethodProtein G-Sepharose™ Chromatography
      Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated on EGF-treated human A431 carcinoma cells.

      Western Blot Analysis:
      0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells (Cohen, B., 1990; Druker, B. J., 1989; Kanakura, Y., 1991).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.

      NOTE: DO NOT FREEZE.
      For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8ºC. Save the supernatant for application.
      Packaging Information
      Material Size1 mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      05-321MG 04053252370953

      Documentation

      Anti-Phosphotyrosine Antibody, clone 4G10® Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-Phosphotyrosine Antibody, clone 4G10® Certificados de análisis

      CargoNúmero de lote
      Anti-Phosphotyrosine, clone 4G10® 3069331
      Anti-Phosphotyrosine, clone 4G10® - 3425585 3425585
      Anti-Phosphotyrosine, clone 4G10® - 4131943 4131943
      Anti-Phosphotyrosine, clone 4G10® - 4265117 4265117
      Anti-Phosphotyrosine, clone 4G10® -2683842 2683842
      Anti-Phosphotyrosine, clone 4G10® -2697411 2697411
      Anti-Phosphotyrosine, clone 4G10® -2716171 2716171
      Anti-Phosphotyrosine, clone 4G10® Monoclonal Antibody 2957952
      Anti-Phosphotyrosine, clone 4G10®_2842146 2842146

      Productos y aplicaciones relacionados

      Alternative Packsize

      Número de referencia Descripción  
      05-321 Anti-Phosphotyrosine Antibody, clone 4G10® Precios y disponibilidad
      05-321X Anti-Phosphotyrosine Antibody, clone 4G10® Precios y disponibilidad

      Alternative Format

      Número de referencia Descripción  
      05-777 Anti-Phosphotyrosine Antibody, recombinant clone 4G10® Precios y disponibilidad
      16-101 Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate Precios y disponibilidad
      16-103 Anti-Phosphotyrosine Antibody, clone 4G10®, Biotin Conjugate Precios y disponibilidad
      16-104 Anti-Phosphotyrosine Antibody, clone 4G10®, FITC conjugate Precios y disponibilidad
      16-105 Anti-Phosphotyrosine Antibody, clone 4G10®, HRP conjugate Precios y disponibilidad
      16-199 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate Precios y disponibilidad
      16-204 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate Precios y disponibilidad
      16-205 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, FITC conjugate Precios y disponibilidad

      Included Positive Control

      Número de referencia Descripción  
      12-302 EGF-Stimulated A431 Cell Lysate Precios y disponibilidad

      Familias de productos

      Phosphotyrosine Signaling Solutions

      Study tyrosine phosphorylation using Millipore's specific and sensitive antibodies, including the popular clone 4G10®, and new 4G10 Platinum, available in various conjugations. See below for more.
      Más información >>

      Phosphotyrosine Antibodies

      Millipore’s phosphotyrosine antibodies, including the popular 4G10 clone, demonstrate sensitivity and detect multiple tyrosine phosphorylations on numerous substrates. See below for pTyr antibodies.
      Más información >>

      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies