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ABC1428-25UG Anti-RecQ1

Detect ATP-dependent DNA helicase Q1 using this rabbit polyclonal Anti-RecQ1, Cat. No. ABC1428-25UG, validated for use in Western Blotting Immunofluorescence and Immunohistochemistry.

Anti-RecQ1: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Apoptosis & Cancer Gene Symbol: RECQL, RECQ1, RECQL1 UniProt Number: P46063
ABC1428-25UG
25 μg  
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      Descripción

      Replacement Information

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IF, ICCRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC1428-25UG
      DescriptionAnti-RecQ1
      Alternate Names
      • ATP-dependent DNA helicase Q1
      • RecQ-like type 1
      • DNA-dependent ATPase Q1
      • RecQ protein-like 1
      Background InformationATP-dependent DNA helicase Q1 (UniProt: P46063; also known as EC:3.6.4.12, DNA helicase, RecQ-like type 1, RecQ1, DNA-dependent ATPase Q1, RecQ protein-like 1) is encoded by the RECQL (also known as RECQ1, RECQL1) gene (Gene ID: 5965) in human. RecQ helicases are a ubiquitous family of DNA unwinding enzymes involved in the maintenance of chromosome stability. They participate in various types of DNA repair, including mismatch repair, nucleotide excision repair and direct repair. Five members of the RecQ family have been reported in human cells: BLM, RECQ1, RECQ4, RECQ5, and WRN. RecQ1 displays high expression in heart, lung, skeletal muscle and kidney. It is also highly expressed in tumor cells and although brain exhibits low level of EecQ1, its expression is dramatically increased in human glioblastoma. Depletion of RecQ1 affects proliferation of glioblastoma cells and causes an increase in the level of DNA damage. RecQ1 depleted tumor cells are shown to be more sensitive to anti-tumor agents, such as hydroxyurea and temozolomide.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing Tris-citrate-phosphate buffer (pH 7.8) with 0.09% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect ATP-dependent DNA helicase Q1 using this rabbit polyclonal Anti-RecQ1, Cat. No. ABC1428-25UG, validated for use in Western Blotting Immunofluorescence and Immunohistochemistry.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Immunocytochemistry
      Application NotesImmunofluorescence Analysis: A representative lot detected RecQ1 in T98G cells after treatment with anti-RECQ1 siRNA or control siRNAs (Mendoza-Maldonado, R., et. al. (2011). Mol Cancer. 10:83).

      Western Blotting Analysis: A representative lot detected RecQ1 in Western Blotting applications (Thangavel, S., et. al. (2015). J Cell Biol. 208(5):545-62).

      Immunohistochemistry Analysis: A representative lot detected RecQ1 in tissues are perilesional sections from multiform glioblastoma, colon carcinoma,lung cancer and thyroid cancer (Mendoza-Maldonado, R., et. al. (2011). Mol Cancer. 10:83).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human ATP-dependent DNA helicase Q1 (RecQ1).
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects ATP-dependent DNA helicase Q1 (RecQ1) in human cells. It targets an epitope within 16 amino acids from the C-terminal end.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • RECQL
      • RECQ1
      • RECQL1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight75 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in IMR90 cell lysate.
      Western Blotting Analysis: 1 µg/mL of this antibody detected RecQ1 in 10 µg of IMR90 human fetal lung cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      ABC1428-25UG 04054839441639