Millipore Sigma Vibrant Logo

04-1550 Anti-SC-35 Antibody, clone 1SC-4F11

Anti-SC-35 Antibody, clone 1SC-4F11 is a Mouse Monoclonal Antibody for detection of SC-35 also known as Protein PR264, Splicing factor SC35 & has been validated in WB, ICC.

Anti-SC-35 Antibody, clone 1SC-4F11: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: SFRS2, PR264, SC-35, SC35, SFRS2A, SRp30b UniProt Number: Q01130
View Products on Sigmaaldrich.com
04-1550
100 µL  
Recuperando precio...
No pudo obtenerse el precio
La cantidad mínima tiene que ser múltiplo de
Maximum Quantity is
Al finalizar el pedido Más información
Ahorró ()
 
—
Solicitar precio
—
Disponibilidad a confirmar Disponibilidad a confirmar
Ingrese cantidad 
Suspendido
Cantidades limitadas disponibles
Debe confirmarse disponibilidad
    El resto: se avisará
      El resto: se avisará
      Se avisará
      Póngase en contacto con el Servicio de Atención al Cliente
      Contact Customer Service

      Ofertas especiales

       

      Póngase en contacto con el Servicio de Atención al Cliente

      Descripción

      Replacement Information

      Ofertas especiales

      100% Performance Guaranteed

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCMAscitesMonoclonal Antibody
      Description
      Catalogue Number04-1550
      DescriptionAnti-SC-35 Antibody, clone 1SC-4F11
      Alternate Names
      • Protein PR264
      • Splicing component, 35 kDa
      • Splicing factor SC35
      • splicing factor, arginine/serine-rich 2
      Background InformationSC-35 is also known as Splicing factor SC35 and Splicing factor, arginine/serine-rich 2. This protein is encoded by the SFRS2 gene and is a member of the splicing factor SR family. SC-35 is involved in pre-mRNA splicing and is found in bodies in the nucleus that are highly enriched in poly(A) RNA. These nuclear bodies are referred to as speckles, SC 35 domains, or splicing factor compartments (SFCs). The purpose of these bodies is still widely disputed. Speckles correspond largely to, if not entirely to, ultra structures called inter-chromatin granule clusters (IGCs). SC3-35 is required for pre-mRNA splicing events and is highly involved in spliceosomal interaction and assembly. SC-35 is necessary for assembly of the ATP-dependent splicing complex. It is also necessary for the ATP-dependent communication between pre-mRNA and U1 and U2 snRNPs.
      References
      Product Information
      FormatAscites
      Control
      • A431 cell lysate
      PresentationUnpurified mouse monoclonal IgG1κ preservative-free ascites.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SC-35 Antibody, clone 1SC-4F11 is a Mouse Monoclonal Antibody for detection of SC-35 also known as Protein PR264, Splicing factor SC35 & has been validated in WB, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: 1:500 dilution from a representative lot detected SC35 in NIH/3T3 cells.
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to human SC35.
      EpitopeUnknown
      Cloneclone 1SC-4F11
      HostMouse
      SpecificityThis antibody recognizes SC35.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SFRS2
      • PR264
      • SC-35
      • SC35
      • SFRS2A
      • SRp30b
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Necessary for the splicing of pre-mRNA. It is required for formation of the earliest ATP-dependent splicing complex and interacts with spliceosomal components bound to both the 5'- and 3'-splice sites during spliceosome assembly. It also is required for ATP-dependent interactions of both U1 and U2 snRNPs with pre-mRNA. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Binds to purine-rich RNA sequences, either 5'-AGSAGAGTA-3' (S=C or G) or 5'-GTTCGAGTA-3'. Can bind to beta-globin mRNA and commit it to the splicing pathway.

      SUBUNIT STRUCTURE: In vitro, binds SFRS1 (ASF/SF2), SNRNP70 and U2AF1 but not U2AF2. Binds SFRS12. Interacts with CCNL1 and CCNL2. Interacts with SFRS2IP.

      SUBCELLULAR LOCATION: Nucleus.

      PTM: Extensively phosphorylated on serine residues in the RS domain.

      SEQUENCE SIMLARITIES: Belongs to the splicing factor SR family.

      Contains 1 RRM (RNA recognition motif) domain.
      Molecular Weight~ 35 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in A431 cell lysate.

      Western Blot Analysis: 1:1,000 dilution of this antibody detected Sc35 on 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      04-1550 04053252376511

      Documentation

      Anti-SC-35 Antibody, clone 1SC-4F11 Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-SC-35 Antibody, clone 1SC-4F11 Certificados de análisis

      CargoNúmero de lote
      Anti-SC-35, clone 1SC-4F11 3168284
      Anti-SC-35, clone 1SC-4F11 2471435
      Anti-SC-35, clone 1SC-4F11 - 2043931 2043931
      Anti-SC-35, clone 1SC-4F11 - 2193339 2193339
      Anti-SC-35, clone 1SC-4F11 - 2272160 2272160
      Anti-SC-35, clone 1SC-4F11 - 2341108 2341108
      Anti-SC-35, clone 1SC-4F11 - 2377620 2377620
      Anti-SC-35, clone 1SC-4F11 - 3190917 3190917
      Anti-SC-35, clone 1SC-4F11 - 3199195 3199195
      Anti-SC-35, clone 1SC-4F11 - 3241220 3241220
      Show more

      Referencias bibliográficas

      Visión general referenciasAplicación Pub Med ID
      B-Myb switches from Cyclin/Cdk-dependent to Jnk- and p38 kinase-dependent phosphorylation and associates with SC35 bodies after UV stress.
      Werwein, E; Dzuganova, M; Usadel, C; Klempnauer, KH
      Cell death & disease  4  e511  2013

      Mostrar resumen       		Immunofluorescence23449447 23449447