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MABN2423-25UG Anti-THIK-2 Antibody, clone N468/37

Anti-THIK-2, clone N468/37, Cat. No. MABN2423, is a mouse monoclonal antibody that detects Potassium channel, subfamily K, member 12 (THIK-2) and is tested for use in Immunohistochemistry (Paraffin).

MABN2423-25UG
25 μg  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberMABN2423-25UG
      DescriptionAnti-THIK-2 Antibody, clone N468/37
      Alternate Names
      • Potassium channel
      • subfamily K
      • member 12
      • MNTK1
      Background InformationMNTK1 (UniProt: Q76M80; also known as Potassium channel, subfamily K, member 12, Tandem pore domain halothane inhibited K channel, THIK-2) is encoded by the Knck12 (also known as Mntk1) gene (Gene ID: 210741) in murine species. THIK-2 is a member of a novel 2P K1 channel subfamily and is an integral component of plasma membrane with six transmembrane domains. Although it displays about 62% sequence homology with THIK-1, it is not active upon heterologous expression, but is shown to form active heteromeric functional channel along with THIK-1 in the plasma membrane. Conductance of THIK-2 is reported to be smaller ( 2.5 pS), however THIK-1-THIK-2 heterodimers display an intermediate single-channel conductance ( 3.5 pS). The N-terminal region of THIK-2 is reported to be responsible for intracellular retention of the homomeric channel in the endoplasmic reticulum and this retention limits the surface expression of THIK-2 channel. THIK-2 is expressed in several tissues including stomach, liver, and kidney and is particularly abundant in the brain. Murine THIK-2 displays about 98% and 97% sequence homology with human and rat THIK-2, respectively. Murine THIK-2 contains two ion transport domains that are localized in amino acids 111-168 and 224-302. THIK-2 protein has an unusual N terminus containing several repeats of proline, arginine, and cysteine residues. (Ref.: Rajan, S., et al. (2001). J. Biol. Chem. 276(10); 7302-7311; Blin, S., et al. (2014). J. Biol. Chem. 289(41); 28202-28212).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-THIK-2, clone N468/37, Cat. No. MABN2423, is a mouse monoclonal antibody that detects Potassium channel, subfamily K, member 12 (THIK-2) and is tested for use in Immunohistochemistry (Paraffin).
      Key Applications
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected THIK-2 in mouse hippocampus tissue sections.
      Biological Information
      ImmunogenA recombinant fragment corresponding to 106 amino acids from the C-terminal, cytoplasmic domain of mouse THIK-2 (MNTK1).
      Epitopecytoplasmic domain
      CloneN468/37
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone N468/37 is a mouse monoclonal antibody that detects Potassium channel, subfamily K, member 12 (THIK-2).
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteMouse, Rat. Predicted to react with Human based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Mntk1
      • Knck12
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight46.95 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in rat cerebellum tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected THIK-2 in rat cerebellum tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABN2423-25UG 04061842667710