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MABN1188 Anti-Tyrosine Hydroxylase Antibody, clone 10F8.2

This Anti-Tyrosine Hydroxylase Antibody, clone 10F8.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Tyrosine Hydroxylase.

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Neuroscience Research Sub-Category: Neuronal & Glial Markers Gene Symbol: Th UniProt Number: P04177
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MABN1188
100 µL  
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      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1188
      ReplacesMAB318
      DescriptionAnti-Tyrosine Hydroxylase Antibody, clone 10F8.2
      Alternate Names
      • TH
      • Tyrosine 3-monooxygenase
      • Tyrosine 3-hydroxylase
      • Tyrosine Hydroxylase
      Background InformationTyrosine 3-monooxygenase (EC 1.14.16.2; UniProt P04177; also known as TH, Tyrosine 3-hydroxylase) is encoded by the Th gene (Gene ID 25085) in rat species. Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines, dopamine and norepinephrine, from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. TH enzymatic activity requires ferrous ions as cofactor and is believed to be regulated by phosphorylation. Six human TH isoforms exist as a result of alternative splicing (UniProt P07101-1 through P07101-6).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2bλ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Tyrosine Hydroxylase Antibody, clone 10F8.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Tyrosine Hydroxylase.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:250 dilution from a representative lot detected tyrosine hydroxylase in rat brain tissue sections.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a C-terminal region sequence of rat tyrosine hydroxylase.
      EpitopeC-terminal region.
      Clone10F8.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 10F8.2 targets a C-terminal epitope present in rat and mouse TH sequence (UniProt P04177 & P24529), as well as in all six human TH spliced isoforms (UniProt P07101).
      IsotypeIgG2bλ
      Species Reactivity
      • Rat
      • Human
      Species Reactivity NoteHuman, Rat. Predicted to react with Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Th
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~50-60 kDa observed. 55.83 kDa (rat), 55.86 kDa (mouse), 58.03/55.48/58.47/55.92/45.21/44.77 kDa (human isoform 1/2/3/4/5/6) calculated (Met1 removed). The multiplet banding pattern observed in rat PC12 lysate most likely represent differentially phosphorylated TH species (Gozal, E., et al. (2005). J. Appl. Physiol. 99(2):642-649).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in PC12 cell lysate.

      Western Blotting Analysis: 0.25 µg/mL of this antibody detected tyrosine hydroxylase in 10 µg of PC12 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABN1188 04055977355437

      Documentation

      Anti-Tyrosine Hydroxylase Antibody, clone 10F8.2 Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Anti-Tyrosine Hydroxylase Antibody, clone 10F8.2 Certificados de análisis

      CargoNúmero de lote
      Anti-Tyrosine Hydroxylase, -Q2696989 Q2696989
      Anti-Tyrosine Hydroxylase, Clone 10F8.2 - 3163461 3163461
      Anti-Tyrosine Hydroxylase, Clone 10F8.2 - 3473485 3473485
      Anti-Tyrosine Hydroxylase, Clone 10F8.2 - 3792015 3792015
      Anti-Tyrosine Hydroxylase, Clone 10F8.2 - 4152037 4152037
      Anti-Tyrosine Hydroxylase, Clone 10F8.2 - 4277707 4277707

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      Número de referencia Descripción  
      AB152 Anti-Tyrosine Hydroxylase Antibody Precios y disponibilidad

      Categorías

      Life Science Research > Antibodies and Assays > Primary Antibodies