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ABN2260-25UG Anti-alpha-synuclein N103 fragment

Anti-alpha-synuclein N103 fragment Antibody, Cat. No. ABN2260, is a highly specific rabbit polyclonal antibody that targets alpha-synuclein and has been tested for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Anti-alpha-synuclein N103 fragment: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
Research Category: Neuroscience Gene Symbol: SNCA, NACP, PARK1 UniProt Number: P37840
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ABN2260-25UG
25 μg  
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      Descripción

      Replacement Information

      Tabla espec. clave

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IHC, IP, WBRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN2260-25UG
      DescriptionAnti-alpha-synuclein N103 fragment
      Alternate Names
      • Non-A beta component of AD amyloid
      • Non-A4 component of amyloid precursor
      • NACP
      Background InformationAlpha-synuclein (UniProt: P37840; also known as Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP) is encoded by the SNCA (also known as NACP, PARK1) gene (Gene ID 6622) in human. Alpha-synuclein is abundantly expressed in the brain and is found in a classic amyloid fibril form within the intra-neuronal Lewy body deposits of Parkinson's disease brains. The physiological function of synucleins is not well understood, but appears to involve membrane interactions, and in particular reversible binding to synaptic vesicle membranes. It may also be involved in the regulation of dopamine release and transport. It is also reported to reduce neuronal responsiveness to various apoptotic stimuli, leading to a decreased caspase-3 activation. Genetic alterations of SNCA gene is reported to result in aberrant polymerization into fibrils, which is associated with several neurodegenerative diseases (synucleinopathies). Alpha-synuclein is also sown to serve as a substrate for asparagine endopeptidase (AEP) that cleaves it at N103 in an age-dependent manner. This cleavage triggers alpha-synuclein aggregation and neurotoxicity, which leads to the loss of dopaminergic neurons. Higher activity of AEP has been shown in subjects with Parkinson s disease and knockout of AEP is reported to ameliorate pathological effects of alpha-synuclein in animal models. Mutations in SNCA gene are also known to Parkinson's disease characterized by bradykinesia, resting tremor, muscular rigidity and postural instability. The pathology of Parkinson s disease involves progressive loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies in surviving neurons in various areas of the brain. (Ref.: Zhang, Z et al., (2017), Nat. Struct. Mol. Biol. 24(8); 632-6420).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS with 0.02 mg/ml sodium azide and 40% glycerol.
      Applications
      ApplicationAnti-alpha-synuclein N103 fragment Antibody, Cat. No. ABN2260, is a highly specific rabbit polyclonal antibody that targets alpha-synuclein and has been tested for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected alpha-synuclein N103 fragment in Western Blotting applications (Zhang, Z., et. al. (2017). Nat Struct Mol Biol. 24(8):632-642).

      Immunoprecipitation Analysis: A representative lot detected alpha-synuclein N103 fragment in Immunoprecipitation applications (Zhang, Z., et. al. (2017). Nat Struct Mol Biol. 24(8):632-642).

      Immunocytochemistry Analysis: A representative lot detected alpha-synuclein N103 fragment in Immunocytochemistry applications (Zhang, Z., et. al. (2017). Nat Struct Mol Biol. 24(8):632-642).

      ELISA Analysis: A representative lot detected alpha-synuclein N103 fragment in ELISA applications (Zhang, Z., et. al. (2017). Nat Struct Mol Biol. 24(8):632-642).

      Immunohistochemistry Analysis: A representative lot detected alpha-synuclein N103 fragment in Immunohistochemistry applications (Zhang, Z., et. al. (2017). Nat Struct Mol Biol. 24(8):632-642).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to nine amino acids that precede the asparagine endopeptidase (AEP)-cleavage site at N103.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects human alpha-synuclein N103 fragment.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SNCA
      • NACP
      • PARK1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~38 kD observed (GFP Fusion protein); 14.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in GFP-alpha synuclein N103.

      Western Blotting Analysis: 2 µg/mL of this antibody detected alpha-synuclein N103 fragment in GFP-alpha synuclein N103.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      ABN2260-25UG 04054839531880