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MABT856-25UG Anti-von Willebrand factor (vWF) Antibody, clone IIIE2.34

Anti-von Willebrand factor, clone IIIE2.34, Cat. No. MABT856, is a mouse monoclonal antibody that detects human von Willebrand factor and has been tested for use in Immunocytochemistry and Western blotting.

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MABT856-25UG
25 μg  
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      Descripción

      Replacement Information
      Description
      Catalogue NumberMABT856-25UG
      DescriptionAnti-von Willebrand factor (vWF) Antibody, clone IIIE2.34
      Alternate Names
      • vWF
      Background Informationvon Willebrand factor (UniProt: P04275; also known as vWF) is encoded by the VWF (also known as F8VWF) gene (Gene ID:7450) in human. vWF is a multimeric plasma glycoprotein that is synthesized by endothelial cells and plays an important in the maintenance of hemostasis. It is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off in the mature form, which is then cleaved into von Willebrand antigen 2 (aa 23-763) and vWF (aa 764-2813). vWF is known to promote adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. vWF also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. Mutations in VWF gene are linked to von Willebrand diseases 1-3 that are common hemorrhagic disorders caused by impaired platelet aggregation. Two isoforms of vWF have been described that are produced by alternative splicing.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Applications
      ApplicationAnti-von Willebrand factor, clone IIIE2.34, Cat. No. MABT856, is a mouse monoclonal antibody that detects human von Willebrand factor and has been tested for use in Immunocytochemistry and Western blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected von Willebrand factor (vWF) in lysates from Human umbilical vein endothelial cells (HUVEC).

      Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected von Willebrand factor (vWF) in Human umbilical vein endothelial cells (HUVEC).
      Biological Information
      ImmunogenProtease fragment of VWF binding to heparin sepharose from human plasma.
      CloneIIIE2.34
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone IIIE2.34 is a mouse monoclonal antibody that detects human von Willebrand factor. It targets an epitope with in the VWFD3 domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • VWF
      • F8VWF
      Purification MethodProtein A purified
      UniProt Number
      Molecular Weight~270 kDa observed, 309.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human platelets cell lysates.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected von Willebrand factor (vWF) in human platelets cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      MABT856-25UG 04054839755231