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SCC624 Lacun.3 Mouse Lung Adenocarcinoma Cell Line

Lacun.3 cells are capable of forming spheroids that can be maintained over multiple passages and display increased cancer stem cell characteristics including increased ALDH activity.

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≥1X10⁶ cells/vial   
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      Descripción

      Replacement Information
      Description
      Catalogue NumberSCC624
      DescriptionLacun.3 Mouse Lung Adenocarcinoma Cell Line
      Alternate Names
      • Lacun-3
      • Lacun3
      Background InformationRecent cancer research often studies a newly characterized type of cell referred to as cancer stem cells (CSCs) or also sometimes termed tumor-initiating cells (TICs.) These cells appear to have significant capabilities to grow, differentiate, generate tumors and resist chemotherapy. Tumor sphere cultures (TSCs) have emerged as a new format of studying cancer as well as understanding CSCs. The spheroid structures of TSCs appear to induce stem-like characteristics evidenced by the increased expression of stem cell markers. This 3D spheroid structure is also a much more accurate way to mimic the behavior of a solid tumor which develops in 3-dimensions and behaves quite differently from a monolayer cell culture. Spheroids are understood to have more tumor-forming capabilities when compared to monolayer cultures, yet the underlying mechanism for these characteristics is still under study. Stem-cell like properties, such as increased aldehyde dehydrogenase activity (ALDH), have already been used to identify CSCs in a variety of cancer types.

      The Lacun.3 cell line was obtained from a chemically induced lung adenocarcinoma which developed in mice.1 This cell line is capable of forming spheroids when cultured in the appropriate media. The spheroid culture can be maintained over multiple passages and displays increased stem-cell characteristics including increased ALDH activity when compared to adherent cultures using the same line. TSC cultured Lacun.3 shows high tumor formation when injected into mice although metastatic capabilities appear to be weakened. While TSC Lacun.3 growth characteristics are slower compared to adherent culture, their chemoresistance capabilities were higher. In addition, a drug that was screened to specifically kill CSCs had much higher success in killing TSC Lacun.3 cells, indicating their CSC phenotype. Furthermore, the Lacun.3 cell line provides a useful model for studying CSCs by spheroid formation.

      Source
      Non-GMO. Cell line is derived from a chemically induced lung adenocarcinoma developed in a mouse model.

      References
      1. Bleau A-M, Zandueta C, Redrado M, Martínez-Canarias S, Larzábal L, Montuenga LM, Calvo A, Lecanda F. 2015. Sphere-derived tumor cells exhibit impaired metastasis by a host-mediated quiescent phenotype. Oncotarget. 6(29):27288–27303. doi:https://doi.org/10.18632/oncotarget.4803. [accessed 2024 Apr 11].
      ‌2. Weiswald L-B, Bellet D, Dangles-Marie V. 2015. Spherical Cancer Models in Tumor Biology. Neoplasia. 17(1):1–15.
      ‌3. Toledo-Guzmán ME, Hernández MI, Gómez-Gallegos ÁA, Ortiz-Sánchez E. 2019. ALDH as a Stem Cell Marker in Solid Tumors. Current Stem Cell Research & Therapy. 14(5):375–388.
      References
      Product Information
      Components
      • ≥1x106 viable Lacun.3 Cells (Catalog No. SCC624). Store in liquid nitrogen.
      Quality LevelMQ100
      Applications
      ApplicationLacun.3 cells are capable of forming spheroids that can be maintained over multiple passages and display increased cancer stem cell characteristics including increased ALDH activity.
      Key Applications
      • Cell Culture
      Application NotesThis product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.
      Biological Information
      HostMouse
      Cell Line Type
      • Cancer Cells
      • Lung cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Each vial contains ≥ 1X106 viable cells.
      • Cells are tested negative for infectious diseases by a Essential CLEAR Panel by Charles River Animal Diagnostic Services.
      • Cells are verified to be of mouse origin and negative for interspecies contamination from human, rat, Chinese hamster, Golden Syrian hamster, and nonhuman primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
      • Cells are negative for mycoplasma contamination.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsLacun.3 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.
      Packaging Information
      Material Size≥1X10⁶ cells/vial
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      SCC624 04065272001410