AAMT001 Sigma-AldrichMitochondrial Complex I Activity Assay Kit

This product has been discontinued.

Product Description

The Mitochondrial Complex I Activity Assay Kit (Cat. No. AAMT001-1KIT) is used to determine the activity of mitochondrial OXPHOS Complex I (NADH dehydrogenase, E.C. 1.6.5.3). The enzyme complex is immunocaptured within the wells of the microplate and activity is determined by following the oxidation of NADH to NAD⁺ and the simultaneous reduction of a dye which leads to increased absorbance at 450 nm.

See Figure 1 at the bottom of this page for the Complex I activity reaction scheme. The oxidation of NADH is linked to the reduction of a dye which has increased absorbance at 450 nm and so appears yellow in color.

25 ml 	Buffer, (72560-25ML)
10 ml	10X Blocking Solution, (72561-10ML)
1 ml	Detergent, (72562-1ML)
1 ea        20X NADH (40 mM), (72563-1EA)
1 ea        100X Dye, (72564-1EA)
1 ea	Microplate, (72565-1EA)

* This kit contains sufficient materials for 96 measurements (one 96-well microplate).

Storage: Store all components at 4°C. After hydration of the 20X NADH and 100X dye with dH₂0, both of these components should be stored be aliquotted and stored at –80°C, as described in the FAQ section of TB554.

This kit contains a 96-well microplate with a monoclonal antibody pre-bound to the wells of the microplate. This plate can be broken into 12 separate 8-well strips for convenience; therefore the plate can be used for up to 12 separate experiments.

The Mitochondrial Complex I Activity Assay Kit contains sufficient materials for 96 measurements (one 96-well microplate).