EAMT003 PDH ELISA Kit

This product has been discontinued.

The Pyruvate Dehydrogenase (PDH) ELISA Kit can be used to determine the amount of PDH protein in a sample. This assay is a 'sandwich' ELISA, where the PDH enzyme is purified and immobilized by an anti-PDH capture antibody pre-coated in the microplate wells. The amount of captured PDH is determined by adding a second (detector) anti-PDH antibody which binds to the captured PDH, followed by binding of an HRP conjugated goat anti-mouse antibody that binds the detector anti-PDH antibody. The detector-bound HRP then changes the colorless HRP development solution to blue and the color intensity (absorbance) is proportional to the amount of PDH captured.

20 ml 	20X Buffer, (72252-20ML)
1 ml	Detergent, (72253-1ML)
1 ml	20X Detector Antibody, (72254-1ML)
1 ml	20 HRP Label, (72255-1ML)
20 ml	HRP Development Solution, (72556-20ML)
1 ea	Microplate, (72257-1EA)
13 ml	5X Stabilizer, (72258-13ML)
10 ml	10X Blocking Solution, (72259-10ML)

* This kit contains sufficient materials for 96 measurements (one 96-well microplate).

Storage: Store the 5X stabilizer at –20°C. Store all other components at 4°C. When stored as recommended, the kit is stable for at least 6 months.

Note: Avoid freeze-thaw cycles cycles of frozen components by making appropriate aliquots if the entire plate is not run in a single 96-well experiment.