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525280 PhosphoDetect™ Anti-Phosphoserine Mouse mAb (16B4)

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      Description
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      This product has been discontinued.





      Recognizes a broad range of serine-phosphorylated proteins in crude extracts from multiple species, requiring either proline or lysine C-terminal to the phosphoserine. Supplied with a positive control.
      Catalogue Number525280
      Brand Family Calbiochem®
      Application Data
      Detection of phosphoserine-containing proteins by immunoblotting. Sample: Whole cell lysate from A431 cells treated with pervanadate (all lanes). Primary antibody: Anti-Phosphoserine Mouse mAb (16B4) (Cat. No. 525280) (1 µg/ml). Detection: chemiluminescence. Available separately: Anti-Phosphoserine (1C8) (Mouse) (Cat. No. 525281), Anti-Phosphoserine (4A3) (Mouse) (Cat. No. 525283), and Anti-Phosphoserine (4A9) (Mouse) (Cat. No. 525284), and Phosphoserine Detection Kit (Cat. No. 525282).

      Detection of phosphoserine-containing antibodies by immunoblotting. Samples: Extract from rabbit muscle*. Primary antibody: Anti-Phosphoserine Mouse mAb (16B4) (Cat. No. 525280) (1 µg/ml). Detection: chemiluminescence. *Positive control supplied with the antibody. Available separately: Anti-Phosphoserine (1C8) (Mouse) (Cat. No. 525281), Anti-Phosphoserine (4A3) (Mouse) (Cat. No. 525283), and Anti-Phosphoserine (4A9) (Mouse) (Cat. No. 525284), and Phosphoserine Detection Kit (Cat. No. 525282).
      References
      Product Information
      FormLyophilized
      Formulation50 µg antibody lyophilized from 2X PBS, PEG, sucrose and control lysate lyophilized from 2X PBS, PEG, sucrose, 10 mM NaF.
      Preservative≤0.1% sodium azide (antibody and control lysate)
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesELISA (0.05 µg/ml)
      Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
      Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
      Application CommentsRecognizes phosphorylated serine residues that are followed by a C-terminal lysine or proline. Note: phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence. Use BSA for membrane blocking and antibody dilutions, do not use milk/casein. For immunoblotting use 5 µl (chemiluminescence) or 20 µl (colorimetric) per lane (mini gel). Recognizes either -Ser-Pro- or -Ser-Lys- sequences that have been phosphorylated on the serine residue. Reacts with human, mouse, rat, chicken, frog, zebrafish, tobacco, maize, arabidopsis, yeast, and bacterial proteins containing phosphoserine. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena mixture of phosphopeptides containing phosphorylated serine, conjugated to KLH
      Clone16B4
      HostMouse
      IsotypeIgM
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 1 ml H₂O. Following reconstitution, aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 year at -70°C or for up to 3 months at 4°C. Thaw aliquots at 37°C. Solubilize rabbit muscle positive control with H₂O; please see vial label for lot-specific reconstitution volume. Add an equal volume of SDS-PAGE sample buffer and incubate at 90°C for 5 min.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      525280 0

      Documentation

      Licencias necesarias

      Título
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      PhosphoDetect™ Anti-Phosphoserine Mouse mAb (16B4) Certificados de análisis

      CargoNúmero de lote
      525280
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-January-2009 JSW
      ApplicationELISA (0.05 µg/ml)
      Immunoblotting (1 µg/ml, colorimetric; 0.1 µg/ml, chemiluminescence)
      Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
      Application Data
      Detection of phosphoserine-containing proteins by immunoblotting. Sample: Whole cell lysate from A431 cells treated with pervanadate (all lanes). Primary antibody: Anti-Phosphoserine Mouse mAb (16B4) (Cat. No. 525280) (1 µg/ml). Detection: chemiluminescence. Available separately: Anti-Phosphoserine (1C8) (Mouse) (Cat. No. 525281), Anti-Phosphoserine (4A3) (Mouse) (Cat. No. 525283), and Anti-Phosphoserine (4A9) (Mouse) (Cat. No. 525284), and Phosphoserine Detection Kit (Cat. No. 525282).

      Detection of phosphoserine-containing antibodies by immunoblotting. Samples: Extract from rabbit muscle*. Primary antibody: Anti-Phosphoserine Mouse mAb (16B4) (Cat. No. 525280) (1 µg/ml). Detection: chemiluminescence. *Positive control supplied with the antibody. Available separately: Anti-Phosphoserine (1C8) (Mouse) (Cat. No. 525281), Anti-Phosphoserine (4A3) (Mouse) (Cat. No. 525283), and Anti-Phosphoserine (4A9) (Mouse) (Cat. No. 525284), and Phosphoserine Detection Kit (Cat. No. 525282).
      DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes phosphoserine-containing proteins. Supplied with a positive control consisting of phosphoproteins isolated from rabbit muscle by affinity chromatography using Fe3+-IDA-Sepharose.
      HostMouse
      Immunogena mixture of phosphopeptides containing phosphorylated serine, conjugated to KLH
      Clone16B4
      IsotypeIgM
      Speciesa broad range of species
      FormLyophilized
      Formulation50 µg antibody lyophilized from 2X PBS, PEG, sucrose and control lysate lyophilized from 2X PBS, PEG, sucrose, 10 mM NaF.
      Preservative≤0.1% sodium azide (antibody and control lysate)
      CommentsRecognizes phosphorylated serine residues that are followed by a C-terminal lysine or proline. Note: phosphoserine detection by monoclonal antibodies is always dependent on the surrounding amino acid sequence. Use BSA for membrane blocking and antibody dilutions, do not use milk/casein. For immunoblotting use 5 µl (chemiluminescence) or 20 µl (colorimetric) per lane (mini gel). Recognizes either -Ser-Pro- or -Ser-Lys- sequences that have been phosphorylated on the serine residue. Reacts with human, mouse, rat, chicken, frog, zebrafish, tobacco, maize, arabidopsis, yeast, and bacterial proteins containing phosphoserine. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the antibody with 1 ml H₂O. Following reconstitution, aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 year at -70°C or for up to 3 months at 4°C. Thaw aliquots at 37°C. Solubilize rabbit muscle positive control with H₂O; please see vial label for lot-specific reconstitution volume. Add an equal volume of SDS-PAGE sample buffer and incubate at 90°C for 5 min.
      Toxicity Standard Handling