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676602 PhosphoDetect™ Anti-VASP (pSer²³⁹) Mouse mAb (16C2)

This PhosphoDetect™ Anti-VASP (pSer²³⁹) Mouse mAb (16C2) is validated for use in ELISA, Immunoblotting, ICC, IP for the detection of VASP (pSer²³⁹).

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: Anti-Vasodilator-stimulated Phosphoprotein, Activated

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      Descripción

      Replacement Information

      Tabla espec. clave

      Species ReactivityHostAntibody Type
      H, MMMonoclonal Antibody
      Description
      OverviewRecognizes the ~46-50 kDa VASP protein phosphorylated at Ser239.
      Catalogue Number676602
      Brand Family Calbiochem®
      SynonymsAnti-Vasodilator-stimulated Phosphoprotein, Activated
      Application Data
      Detection of human VASP, phosphorylated on Ser239, by immunoblotting. Samples: Whole cell extracts from MDA-MB-231 cells left untreated (lane 1) or Forskolin treated cells (lane 2). Primary antibody: PhosphoDetect™ Anti-VASP (pSer239) Mouse mAb (16C2) (Cat. No. 676602) (0.5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesReinhard, M., et al. 1999. In: Guidebook to the Cytoskeletal and Motor Proteins (Eds. Kreis, T., and Vale, R.), Oxford University Press, Oxford, 168.
      Schwarz, U.R., et al. 1999. Thromb. Haemost. 82, 1145.
      Smolenski, A., et al. 1998. J. Biol. Chem. 273, 20029.
      Product Information
      FormLyophilized
      FormulationLyophilized from 2X PBS, PEG, sucrose.
      Positive controlVanadate-treated A431 cells or 8-pCPT-treated platelets
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesELISA (0.05 µg/ml)
      Immunoblotting (0.1 µg/ml, chemiluminescence; 1 µg/ml, colorimetric)
      Immunocytochemistry (1-10 µg/ml)
      Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
      Application CommentsDoes not recognize the non-phosphorylated form of VASP. Suitable for monitoring the phosphorylation state of VASP Ser239 as well as PKA/PKG activity. For immunocytochemistry, SNP or 8-pCPT-cGMP treated platelets are fixed with 4% paraformaldehyde in PBS for 15 min. on ice, then permeabilized with 0.2% Triton® X-100 detergent in PBS for 10 min at room temperature. For immunoblotting, block with 1% hemoglobin in PBS, 0.3% Triton® X-100 detergent, and 0.05% Tween®-20 detergent. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³⁹ phosphorylation site of human VASP, conjugated to KLH
      ImmunogenHuman
      Clone16C2
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute with 1 ml of dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      676602 0

      Documentation

      PhosphoDetect™ Anti-VASP (pSer²³⁹) Mouse mAb (16C2) Ficha datos de seguridad (MSDS)

      Título

      Ficha técnica de seguridad del material (MSDS) 

      Referencias bibliográficas

      Visión general referencias
      Reinhard, M., et al. 1999. In: Guidebook to the Cytoskeletal and Motor Proteins (Eds. Kreis, T., and Vale, R.), Oxford University Press, Oxford, 168.
      Schwarz, U.R., et al. 1999. Thromb. Haemost. 82, 1145.
      Smolenski, A., et al. 1998. J. Biol. Chem. 273, 20029.
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-August-2007 RFH
      SynonymsAnti-Vasodilator-stimulated Phosphoprotein, Activated
      ApplicationELISA (0.05 µg/ml)
      Immunoblotting (0.1 µg/ml, chemiluminescence; 1 µg/ml, colorimetric)
      Immunocytochemistry (1-10 µg/ml)
      Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
      Application Data
      Detection of human VASP, phosphorylated on Ser239, by immunoblotting. Samples: Whole cell extracts from MDA-MB-231 cells left untreated (lane 1) or Forskolin treated cells (lane 2). Primary antibody: PhosphoDetect™ Anti-VASP (pSer239) Mouse mAb (16C2) (Cat. No. 676602) (0.5 µg/ml). Detection: chemiluminescence.
      DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46-50 kDa VASP protein phosphorylated at Ser239.
      BackgroundVASP is a proline-rich substrate of PKA and PKG. It is involved in the enhancement of actin filament formation and has been shown to bind profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. VASP is expressed in most mammalian tissues, with particularly high expression in platelets.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser²³⁹ phosphorylation site of human VASP, conjugated to KLH
      Clone16C2
      IsotypeIgG₁
      Specieshuman, mouse
      Positive controlVanadate-treated A431 cells or 8-pCPT-treated platelets
      FormLyophilized
      FormulationLyophilized from 2X PBS, PEG, sucrose.
      Preservative≤0.1% sodium azide
      CommentsDoes not recognize the non-phosphorylated form of VASP. Suitable for monitoring the phosphorylation state of VASP Ser239 as well as PKA/PKG activity. For immunocytochemistry, SNP or 8-pCPT-cGMP treated platelets are fixed with 4% paraformaldehyde in PBS for 15 min. on ice, then permeabilized with 0.2% Triton® X-100 detergent in PBS for 10 min at room temperature. For immunoblotting, block with 1% hemoglobin in PBS, 0.3% Triton® X-100 detergent, and 0.05% Tween®-20 detergent. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute with 1 ml of dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot, flash-freeze in liquid nitrogen, and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesReinhard, M., et al. 1999. In: Guidebook to the Cytoskeletal and Motor Proteins (Eds. Kreis, T., and Vale, R.), Oxford University Press, Oxford, 168.
      Schwarz, U.R., et al. 1999. Thromb. Haemost. 82, 1145.
      Smolenski, A., et al. 1998. J. Biol. Chem. 273, 20029.

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      Categorías

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