71114 MilliporePopCulture^® His•Mag™ Purification Kit

This product has been discontinued.

The PopCulture® His•Mag™ Purification Kit is designed for purification of His•Tag® fusion proteins directly from E. coli cultures without harvesting cells. The procedure combines PopCulture total culture extraction with magnetic affinity purification using His•Mag Agarose Beads.

PopCulture Reagent is a detergent-based concentrate that can be added directly to cultures of E. coli to effectively extract proteins without the need for centrifugation. Use of His•Mag Agarose Beads enables the entire procedure to be carried out in a single tube without using columns or centrifugation.

The PopCulture His•Mag Purification Kit combines PopCulture Reagent, His•Mag Agarose Beads, corresponding buffers, and rLysozyme™ Solution. This kit enables the processing of 40 × 3-ml cultures with yields up to 375 µg His•Tag® fusion protein per 3-ml culture, based on bead binding capacity. The kit is compatible with the Novagen Magnetight™ Separation Stand. For 96-well processing using PopCulture, try the RoboPop™ His•Mag Purification Kit.

• Binding capacity of 5 mg/ml
• Ni-charged IDA magnetic agarose form with 3 µm average bead size
• No need to separate cells from culture medium: use 25 µl settled beads per milliliter culture (50 µl 50% v/v suspension)
• No need to mechanically disrupt cells
• No need to clarify cell extracts prior to purification
• Direct affinity adsorption of target proteins to resin from the total culture extract
• Ability to rapidly perform the entire cell growth and purification process in a single tube or well