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Unidades de filtración Steritop™

Las unidades de filtración Steritop para utilizar sobre botellas tienen una amplia superficie para la filtración esterilizante y rápida de fluidos acuosos.

Las unidades de filtración Steritop para utilizar sobre botellas tienen una amplia superficie para la filtración esterilizante y rápida de fluidos acuosos en botellas de cuello estrecho (33 mm) o de cuello ancho (45 mm). Una pequeña pestaña en el interior del embudo asegura un prefiltro opcional durante la filtración. Para mayor protección contra la contaminación, las roscas para conectar la botella han sido ligeramente escondidas en caso de que el embudo toque accidentalmente una superficie de trabajo.

Descripción

Especificaciones

Información para pedidos

Unidades con membrana Millipore Express PLUS (PES) de 0,22 µm de caudal elevado y baja adsorción proteica para la esterilización de soluciones acuosas*Borrar Clasificación y Filtrado Show Filter
Número de referenciaDescripciónCapacidad del embudoConexión de salidaTamaño de envase
SCGPS01REUnidad Steritop de filtración a vacío sobre botella 150 mL Rosca de 33 mm 12 Precios y disponibilidad
SCGPS02REUnidad de filtración Steritop-GP; 0,22 µm, poliétersulfona, 250 ml, 33 mm, esterilizada por radiación 250 mL Rosca de 33 mm 12 Precios y disponibilidad
SCGPS05REUnidad de filtración Steritop-GP; 0,22 µm, poliétersulfona, 500 ml, 33 mm, esterilizada por radiación 500 mL Rosca de 33 mm 12 Precios y disponibilidad

* Estos productos han sido ensayados y validados para usar en aplicaciones de investigación con células madre. Para determinar sus efectos en el crecimiento o diferenciación de células madre de ratón, se han utilizado tres lotes de unidades Steritop-GP para filtrar medios de cultivo con LIF. Una vez filtrado, este medio se utilizó para pasar las células madre de ratón cinco veces a fin de verificar que la filtración con las unidades Steritop-GP no afecta a la pluripotencialidad de las células madre de ratón.

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Documentation

Preguntas frecuentes

PreguntaRespuesta
Are Stericup and Steritop units autoclavable?No. The highest temperature that the product can be used at is 50C ( 122F ).
Can I bubble point my Stericup/top?No. The unit is not designed to handle the pressures required to perform a bubble point test. It is designed to be run under vacuum only (15 psi max). Bubble points for any of the membranes in the Stericups/tops need to be run at pressures ranging from 22 psi to 57psi.
Do you have a filter for 100% serum?Yes, you can filter serum with Millipore Express membrane. The Express membrane was designed for this application and offers fast filtration time. Millipore Express is available in a large number of filter devices including Millex syringe filters, Stericup and Steritop vacuum devices, Sterivac for large volumes of media and Steriflip for 50 mls.
For tissue culture media filtration which membrane should I use?The best membrane for this application is the Millipore Express membrane. It was designed specifically to filter tissue culture media with serum. It is low protein binding, has fast flow and high throughput (which is also called filter capacity).
I want to sterilize 100% ethanol. Are any of the Stericups or Steritops compatible with ethanol?The funnel and receiver flask is made of polystyrene. Any molded polystyrene will craze in pure ethanol and could structurally fail depending on the forces being applied to it. The funnel could handle the short exposure time but the receiver would craze eventually. Therefore we would not recommend the Stericup with 100% ethanol. The Steritop, if you keep the exposure time to the funnel minimal, should be fine. As you dilute the alcohol the compatibility improves.

Both Express and Durapore membranes are compatible. MCE is not.
I will be filtering tissue culture media with your Stericup/top and am concerned that wetting agents in the filter could effect my cells. Which membrane type would you recommend to avoid this?All of Millipore's Tissue Culture Media filtration sterilization devices contain membranes that are wetting agent (surfactant ) free.
Is the Express membrane surfactant free?Yes, the Express membrane is surfactant free. It also has a very low extractables specification - less then 1.5% by weight.
When I use my Stericup/top, sometimes some of my sterilized solution gets pulled into my vacuum line. How can I eliminate this from happening?Attach your vacuum tubing and turn the vacuum on before adding the liquid to the Stericup/top. This eliminates any initial solution that flowed through the membrane before starting the vacuum from being pulled into the vacuum line.
What is the diameter of the membrane in the Stericup and Steritop units?All of the cups and tops currently have a 70mm diameter membrane, which has 43 cm2 of surface area.
Why use a vacuum driven filter rather than a pressure driven filter?Unlike positive pressure filtration, vacuum driven filtration requires only a vacuum source. It does not require pressure vessels or peristaltic tubing pumps. Stericup and Steritop units are pre-sterilized and disposable making setup and cleanup easy!
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Categorías

Life Science Research > Cell Culture and Systems > Sterile Filtration Devices