70042 Sigma-AldrichT7Select^® 1-2a DNA

This product has been discontinued.

The T7Select1-2a-c vectors are protein display vectors based on bacteriophage T7. The vector displays an average of 0.1–1 copies of peptides and proteins up to 900 aa in size on the surface of the T7 capsid. Target sequences are fused to the C-terminus of the 10B capsid protein near amino acid 348. T7Select1 phage are grown on a complementing host (BLT5403) that supplies large amounts of the 10A capsid protein from a plasmid. Capsids thus contain mostly 10A protein, along with 0.1–1 copy of 10B fusion protein per virion. The linear genome of T7Select1-2a is 37,245 bp in size. Unique restriction sites are shown on the vector map (TB182).




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