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662087 UCH-L1, His•Tag® , Mouse, Recombinant, E. coli

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.

Sinónimos: Ubiquitin C-terminal Hydrolase L1, Ubiquitin thioesterase L1

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      Descripción

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      Full-length, recombinant, mouse UCHL-1 fused at the N-terminus to His•Tag® sequence and expressed in E. coli. UCH-L1 is a member of the low molecular weight UCHs that processes ubiquitin precursors and ubiquitinated proteins to generate monomeric ubiquitin. The expression of UCH-L1 is highly specific to cells of the neuroendocrine system and their tumors. UCH-L1 variants (Ile93Met) have been linked to Parkinson disease and may participate in processes critical for neuronal health. The active enzyme is useful in the study of ubiquitin regulation and inhibitor screening.
      Catalogue Number662087
      Brand Family Calbiochem®
      SynonymsUbiquitin C-terminal Hydrolase L1, Ubiquitin thioesterase L1
      References
      ReferencesKurihara, LJ., et al. 2001. Hum. Mol. Genet. 10, 1963.
      Johnston, S.C., et al. 1999. EMBO. J. 18, 3877.
      Saigoh, K., et al. 1999. Nat. Genet. 23, 47.
      Dang, L.C. et al. 1998. Biochem. 37, 1868.
      Leroy, E., et al.1998. DNA. Res. 5, 397.
      Day, I.N., et al. 1990. Biochem J. 268, 521.
      Mayer, A.N., and Wilkinson, K.D. 1989. Biochem. 28, 166.
      Pickart, C.M., et al. 1985. J. Biol. Chem. 260, 7903.
      Product Information
      FormulationIn 50 mM HEPES, 2 mM DTT, pH 8.0.
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      662087 0

      Documentation

      UCH-L1, His•Tag® , Mouse, Recombinant, E. coli Certificados de análisis

      CargoNúmero de lote
      662087

      Referencias bibliográficas

      Visión general referencias
      Kurihara, LJ., et al. 2001. Hum. Mol. Genet. 10, 1963.
      Johnston, S.C., et al. 1999. EMBO. J. 18, 3877.
      Saigoh, K., et al. 1999. Nat. Genet. 23, 47.
      Dang, L.C. et al. 1998. Biochem. 37, 1868.
      Leroy, E., et al.1998. DNA. Res. 5, 397.
      Day, I.N., et al. 1990. Biochem J. 268, 521.
      Mayer, A.N., and Wilkinson, K.D. 1989. Biochem. 28, 166.
      Pickart, C.M., et al. 1985. J. Biol. Chem. 260, 7903.
      Ficha técnica

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-July-2009 JSW
      SynonymsUbiquitin C-terminal Hydrolase L1, Ubiquitin thioesterase L1
      DescriptionFull-length, recombinant, mouse UCHL-1 fused at the N-terminus to His•Tag® sequence and expressed in E. coli. UCH-L1 is a member of the low molecular weight UCHs that processes ubiquitin precursors and ubiquitinated proteins to generate monomeric ubiquitin. The expression of UCH-L1 is highly specific to cells of the neuroendocrine system and their tumors. UCH-L1 variants (Ile93Met) have been linked to Parkinson disease and may participate in processes critical for neuronal health. The active enzyme is useful in the study of ubiquitin regulation and inhibitor screening.
      FormulationIn 50 mM HEPES, 2 mM DTT, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Typical enzyme concentration required to support in vitro substrate hydrolysis is 10-50 nM; pre-incubation with 10 mM DTT for 15 min is recommended to achieve maximum activity.
      Purity≥95% by SDS-PAGE
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesKurihara, LJ., et al. 2001. Hum. Mol. Genet. 10, 1963.
      Johnston, S.C., et al. 1999. EMBO. J. 18, 3877.
      Saigoh, K., et al. 1999. Nat. Genet. 23, 47.
      Dang, L.C. et al. 1998. Biochem. 37, 1868.
      Leroy, E., et al.1998. DNA. Res. 5, 397.
      Day, I.N., et al. 1990. Biochem J. 268, 521.
      Mayer, A.N., and Wilkinson, K.D. 1989. Biochem. 28, 166.
      Pickart, C.M., et al. 1985. J. Biol. Chem. 260, 7903.