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14-687M p38α/SAPK2a (T106M) Protein, active, 250 µg

Active, N-terminal GST-tagged, recombinant, human, full-length p38α/SAPK2a containing the mutation T106M. Activated using DD-MKK6 & repurified using glutathione-agarose. For use in Kinase Assays.

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14-687M
250 µg  Also available in 10 μg size (cat#14-687) and in bulk (cat#14-687-K).
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      Descripción

      Replacement Information
      Description
      Catalogue Number14-687M
      Brand Family Upstate
      Trade Name
      • Upstate
      Descriptionp38α/SAPK2a (T106M) Protein, active, 250 µg
      OverviewN-terminal GST-tagged, recombinant, human, full-length p38α/SAPK2a containing the mutation T106M. Activated using DD-MKK6 (14-537) and repurified using glutathione- agarose.

      Contains the gatekeeper mutation T160M in the ATP-binding domain. This mutation increases resistance to SB203580 as compared to the wild type sequence.
      References
      Product Information
      Quality LevelMQ300
      Applications
      ApplicationActive, N-terminal GST-tagged, recombinant, human, full-length p38α/SAPK2a containing the mutation T106M. Activated using DD-MKK6 & repurified using glutathione-agarose. For use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      SourceHuman, full-length p38α/SAPK2a containing the mutation T106M. Expressed in E. coli.
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
      Gene Symbol
      • MAPK14
      • p38
      • CSBP
      • PRKM15
      • PRKM14
      • RK
      • SAPK2A
      • CSBP1
      • CSPB1
      • Mxi2
      • p38ALPHA
      • MXI2
      • EXIP
      • CSBP2
      Protein Targetp38α/SAPK2a
      Purification MethodGlutathione-agarose
      Target Sub-FamilyCMGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q16539 # Responds to activation by environmental stress, pro- inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
      COFACTOR: Magnesium.
      SIZE: 360 amino acids; 41293 Da
      SUBUNIT: Binds to a kinase interaction motif within the protein tyrosine phosphatase, PTPRR. This interaction retains MAPK14 in the cytoplasm and prevents nuclear accumulation. Interacts with SPAG9 (By similarity). Interacts with NP60.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity).
      TISSUE SPECIFICITY: Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.DOMAIN:SwissProt: Q16539 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme. & Phosphorylated upon DNA damage, probably by ATM or ATR.
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight67.7kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by phosphorylation of MBP (13-104).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C from date of shipment
      Packaging Information
      Material Size250 µg
      Material PackageAlso available in 10 μg size (cat#14-687) and in bulk (cat#14-687-K).
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      14-687M 04053252824241

      Documentation

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      14-687 p38α/SAPK2a (T106M) Protein, active, 10 µg Precios y disponibilidad

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      Categorías

      Life Science Research > Proteins and Enzymes > Purified Kinases
      Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases