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71237 pIEx™-1 Ek/LIC Vector Kit

71237
  
Purchase on Sigma-Aldrich

Descripción

Replacement Information
Description
Overview

This product has been discontinued.





The pIEx™-1 Ek/LIC Vector is designed to allow rapid, high-level protein expression in insect cells without the time-consuming process of creating a recombinant baculovirus. This vector features the hr5 enhancer and the IE1 (immediate early) promoter to direct expression in insect cells. This promoter/enhancer combination recruits endogenous insect cell transcription machinery, thereby avoiding baculovirus infection and the associated cytotoxic effects. Cloning sites are available for producing fusion proteins also containing cleavable His•Tag® and S•Tag™ sequences for detection, purification and quantification. A C-terminal HSV•Tag® sequence serves as a cloning option to permit detection of target protein with the HSV•Tag Monoclonal Antibody.

Catalogue Number71237
Brand Family Novagen®
SynonymsInsectDirect™ , pIEx™-1 Ek/LIC Vector Kit
References
Product Information
Components
DeclarationpIEx is a trademark of EMD Biosciences, Inc., Madison, WI USA
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage ≤ -70°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Número de referencia GTIN
71237 0

Documentation

pIEx™-1 Ek/LIC Vector Kit Certificados de análisis

CargoNúmero de lote
71237

Folleto

Cargo
Insect Cell Expression
Radiance! Multisystem Protein Expression

Citas

Título
  • Kathryn H. Loomis, et al. (2005) InsectDirectTM System: rapid, high-level protein expression and purication from insect cells. Journal of Structural and Functional Genomics 6, 189-194.
  • Protocolos de usuario

    Cargo
    TB163 Ek/LIC Cloning Kits

    Mapa vectorial

    Cargo
    TB349VM pIEx™-1 Vector Map