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71729 pIEx/Bac™-1 Ek/LIC Vector Kit - Novagen

pIEx/Bac™-1 Ek/LIC Vector Kit - Novagen: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
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      Descripción

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      The pIEx/Bac™-1 Ek/LIC vector is an Ek/LIC prepared version of pIEx/Bac-1. The pIEx/Bac™ vectors are dual-purpose vectors that offer unique flexibility for cloning and expression of proteins in Spodoptera-derived insect cells. These vectors contain the Autographa californica nuclear polyhedrosis virus (AcNPV) enhancer/immediate early promoter combination (hr5/ie1) for plasmid-mediated and early baculovirus expression, and the AcNPV p10 very late promoter for robust baculovirus-mediated expression. All pIEx/Bac plasmids carry AcNPV-derived virus regions to mediate recombination at the polh locus. The pIEx/Bac-1 vector contains an N-terminal 8 aa Strep•Tag® II coding sequence followed by an enterokinase protease cleavage site and a multiple cloning site, with the option to include a C-terminal His•Tag® coding sequence with 10 histidines.
      Catalogue Number71729
      Brand Family Novagen®
      References
      Product Information
      •20 µg (40 µl) 0.5 µg/µl pIEx/Bac-1 Ek/LIC DNA
      •50 µlT4 DNA Polymerase Buffer
      •5 µg (10 µl) 0.5 µg/µl pIEx/Bac Ek/LIC Positive Expression Control
      •25 UT4 DNA Polymerase, LIC-qualified
      •100 µl100 mM DTT
      •40 µl25 mM dATP
      •50 µl25 mM EDTA
      •1.5 ml Nuclease-free Water
      •1 pkgNovaBlue GigaSingles Competent Cells
      •2 mlSOC Medium
      •10 µlTest Plasmid
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      71729 0