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  • The retention of extracellular matrix proteins and angiogenic and mitogenic cytokines in a decellularized porcine dermis. 20576289

    Decellularized dermis materials demonstrate considerable utility in surgical procedures including hernia repair and breast reconstruction. A new decellularized porcine dermis material has been developed that retains many native extracellular matrix (ECM) proteins and cytokines. This material has substantial mechanical strength with maximum tensile strength of 141.7 +/- 85.4 (N/cm) and suture pull through strength of 47.0 +/- 14.0 (N). After processing, many ECM proteins remained in the material including collagen III, collagen IV, collagen VII, laminin and fibronectin. Glycosaminoglycans, including hyaluronic acid, were also preserved. Among several cytokines whose levels were quantified, more vascular endothelial growth factor (VEGF) and transforming growth factor beta (TGF-beta) were retained within this material than in comparable decellularized dermis materials. The retention of bioactivity was demonstrated in a cell culture assay. Because this decellularized porcine dermis material both retains significant strength and has substantial biological activity, it may promote rapid integration and repair in clinical applications.
    Tipo de documento:
    Referencia
    Referencia del producto:
    MAB1926

  • A rapid methodology for the isolation of intermediate-density lipoprotein: characterization of lipid composition and apoprotein content. 15698598

    BACKGROUND: Intermediate-density lipoprotein (IDL) is a structurally related precursor of low-density lipoprotein (LDL). Although found in significantly lower levels, extensive evidence suggests that IDL shares LDL's capacity to promote atherosclerosis. To assist further investigation into IDL's composition and physiological relevance, we have established a rapid method to extract IDL from plasma. METHODS: IDL was isolated from plasma by sequential floatation ultracentrifugation in 3 h, a significantly shorter isolation time than previously published methods. Apoproteins (apo) B100, CIII, and E, together with the level of albumin contamination, were quantified using single radial immunodiffusion. The lipid composition of IDL was measured using automated enzyme assays. RESULTS: The percent recovery of lipid from all lipoprotein fractions (VLDL+IDL+LDL+HDL) was 97.0+/-4.9% when compared to total plasma lipid. IDL had a reduced concentration of apo CIII, apo E, triglyceride, and free cholesterol, and had a higher concentration of apo B100, cholesterol ester, and phospholipid when compared to VLDL. Pure IDL migrated in advance of LDL during agarose electrophoresis. CONCLUSIONS: This rapid technique minimizes damage to the integrity of IDL and yields sufficient quantities to allow accurate assessment of composition and susceptibility to in vitro oxidation, and thus facilitates further investigation of IDL in the development of atherosclerosis.
    Tipo de documento:
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    Referencia del producto:
    AB821

  • The histone acetyltransferase MOF activates hypothalamic polysialylation to prevent diet-induced obesity in mice. 25161885

    Overfeeding causes rapid synaptic remodeling in hypothalamus feeding circuits. Polysialylation of cell surface molecules is a key step in this neuronal rewiring and allows normalization of food intake. Here we examined the role of hypothalamic polysialylation in the long-term maintenance of body weight, and deciphered the molecular sequence underlying its nutritional regulation. We found that upon high fat diet (HFD), reduced hypothalamic polysialylation exacerbated the diet-induced obese phenotype in mice. Upon HFD, the histone acetyltransferase MOF was rapidly recruited on the St8sia4 polysialyltransferase-encoding gene. Mof silencing in the mediobasal hypothalamus of adult mice prevented activation of the St8sia4 gene transcription, reduced polysialylation, altered the acute homeostatic feeding response to HFD and increased the body weight gain. These findings indicate that impaired hypothalamic polysialylation contribute to the development of obesity, and establish a role for MOF in the brain control of energy balance.
    Tipo de documento:
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    Referencia del producto:
    Múltiplo
    Nombre del producto:
    Múltiplo
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