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  • Anti-XPD, clone 22TF2-2F6 - 3532356

    Tipo de documento:
    Certificado de análisis
    Número de lote:
    3532356
    Referencia del producto:
    MABE1819-100UG
    Nombre del producto:
    Anti-XPD Antibody, clone 22TF2-2F6

  • Production and characterization of monoclonal antibodies to estrogen-related receptor alpha (ERRα) and use in immunoaffinity chromatography. 22565152

    Estrogen-related receptor alpha (ERRα) is an orphan nuclear receptor whose elevated expression is thought to contribute to breast, colon, and ovarian cancers. In order to investigate the role of ERRα in human disease, there is a need for immunological reagents suitable for detection and purification of ERRα. We expressed recombinant human ERRα in Escherichia coli, purified the protein, and used it to generate monoclonal antibodies (mAbs) to ERRα. Nine high-affinity mAbs were chosen for their abilities to detect overexpressed ERRα in enzyme-linked immunosorbent assays (ELISAs) and Western blots, after which isotyping and preliminary epitope mapping was performed. The mAbs were all IgG subtypes and reacted with several different regions of full-length ERRα. A majority of the mAbs were found to be useful for immunoprecipitation of ERRα, and several could detect DNA-bound ERRα in electrophoretic mobility supershift assays (EMSAs) and chromatin immunoprecipitation (ChIP). The suitability of mAbs to detect ERRα in immunofluorescence assays was assessed. One mAb in particular, 2ERR10, could specifically detect endogenous ERRα in mammary carcinoma cells. Finally, we performed assays to screen for mAbs that gently release ERRα in the presence of a low-molecular-weight polyhydroxylated compound (polyol) and nonchaotropic salt. Using gentle immunoaffinity chromatography, we were able to isolate ERRα from mammalian cells by eluting with a polyol-salt solution. Our characterization studies show that these monoclonal antibodies perform well in a variety of biochemical assays. We anticipate that these novel reagents will prove useful for the detection and purification of ERRα in research and clinical applications.
    Tipo de documento:
    Referencia
    Referencia del producto:
    Múltiplo
    Nombre del producto:
    Múltiplo

  • Anti-ERR alpha, clone 2ERR10 - Q3286550

    Tipo de documento:
    Certificado de análisis
    Número de lote:
    Q3286550
    Referencia del producto:
    MABE1891-100UG
    Nombre del producto:
    Anti-ERR alpha Antibody, clone 2ERR10

  • Anti-TAF-15, clone 12F11 - Q3206615

    Tipo de documento:
    Certificado de análisis
    Número de lote:
    Q3206615
    Referencia del producto:
    MABE1918-100UG
    Nombre del producto:
    Anti-TAF-15 Antibody, clone 12F11
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