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70186 pT7Blue-2 Blunt Vector (linearized) - Novagen

Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
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      Descripción

      Replacement Information
      Description
      Overview

      This product has been discontinued.





      The pT7Blue-2 vector features the pUC19 backbone (including high-copy number origin of replication and lac sequences), f1 origin of replication and modified multiple cloning region designed for optimal expression of cloned inserts by in vitro translation. The multiple cloning region contains a T7 promoter upstream of the Xenopus β-globin 5′ UTR translation enhancer and S•Tag™ sequences, followed by an enterokinase cleavage site and extensive region of cloning sites. An SP6 promoter is placed within the multiple cloning sites to allow the synthesis of essentially unfused RNA from DNA inserted downstream.

      Catalogue Number70186
      Brand Family Novagen®
      References
      References

      Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.
      Product Information
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      Safety Information according to GHS
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      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
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      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      70186 0

      Documentation

      pT7Blue-2 Blunt Vector (linearized) - Novagen Certificados de análisis

      CargoNúmero de lote
      70186

      Referencias bibliográficas

      Visión general referencias

      Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.

      Protocolos de usuario

      Cargo
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB183 Perfectly Blunt® Cloning Kits

      Secuencia vectorial

      Cargo
      pT7Blue-2 Sequence

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      PCR and Cloning

      Categorías

      Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Cloning > Cloning Vectors