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70174 pT7Blue Blunt Vector (linearized) - Novagen

pT7Blue Blunt Vector (linearized) - Novagen: Ficha de datos de seguridad (MSDS o SDS), certificado de análisis y de calidad (CoA y CoQ), expedientes, folletos y otros documentos disponibles.
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      Descripción

      Replacement Information
      Description
      Overview

      The pT7Blue vector features the pUC19 backbone (including high-copy number origin of replication and lac sequences), a T7 promoter, f1 origin of replication, and modified multiple cloning region. The multiple cloning region contains an EcoR V site used for T-cloning flanked by an Nde I site, which allows inserts to be conveniently subcloned into the Nde I site of many pET vectors.
      Catalogue Number70174
      Brand Family Novagen®
      Features and benefits
      • No restriction enzymes or special primers
      • Compatible with any DNA polymerase
      • Independent of 3ʹ-dA addition or sequence context of DNA ends
      • Blue/white screening with seven different vectors, including pETBlue expression vectors
      • Simple protocol takes less than one hour* from PCR product to plating transformants

      * All times listed refer to kits using NovaBlue Singles™ Competent Cells and ampicillin or chloramphenicol selection. Giga kits, which use NovaBlue GigaSingles™ Competent Cells, require one hour for outgrowth.
      References
      References

      Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.
      Product Information
      Components
      Quality LevelMQ100
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de referencia GTIN
      70174 0

      Documentation

      pT7Blue Blunt Vector (linearized) - Novagen Certificados de análisis

      CargoNúmero de lote
      70174

      Referencias bibliográficas

      Visión general referencias

      Novy, R.E., et al. 1996. inNovations 6, 7. Clark, J.M. 1988. Nucleic Acids Res. 16, 9677. Brownstein, J.M., et al. 1996. BioTechniques 20, 1004. Magnuson, V.L., et al. 1996. BioTechniques 21, 700. Hu, G. 1993. DNA and Cell Biology 12, 763.

      Citas

      Título
    • Ekaterina Bogdanova, et al. (2008) Transcription regulation of the type II restriction-modification system AhdI. Nucleic Acids Research 36, 1429-1442.
    • Miho Hirabayashi, et al. (2005) Transformation of skeletal muscle from fast- to slow-twitch during acquisition of cold tolerance in the chick. Endocrinology 146, 399-405.
    • J. K. Jang, T. Rahman and K. S. McKim. (2005) The kinesinlike protein subito contributes to central spindle assembly and organization of the meiotic spindle in Drosophila oocytes. 16, 4684-4694.
    • Rahul Pal, et al. (2005) Evidence for multiple shared antigenic determinants within Ro60 and other lupus-related ribonucleoprotein autoantigens in human autoimmune responses. Journal of Immunology 175, 7669-7677.
    • Dana R. Rundle, et al. (2004) Myristoyl-CoA: protein N-myristoyltransferases: isoform identification and gene expression in retina. Molecular Vision 10, 177-185.
      		•

      Protocolos de usuario

      Cargo
      TB053 Academic and Non-profit Laboratory Assurance Letter
      TB183 Perfectly Blunt® Cloning Kits

      Mapa vectorial

      Cargo
      TB017VM pT7Blue Vector Map

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      Categorías

      Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Cloning > Cloning Vectors