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AB5830 Anti-8-Hydroxydeoxyguanosine Antibody

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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, IH(P)GtSerumPolyclonal Antibody
      Catalogue NumberAB5830
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-8-Hydroxydeoxyguanosine Antibody
      Alternate Names
      • 8-OHdG
      • 8-hydroxy-2'-deoxyguanosine
      Background Information8-Hydroxydeoxyguanosine (8OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer's disease.

      8OHdG has become increasing popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA. Biomonitoring in humans has demonstrated that 8OHdG can be excreted in the urine, and that a significant increase is caused by exposure to tobacco smoke and ionizing radiation. Because 8OHdG is so well correlated with oxidative stress and damage to DNA, which leads to degenerative disease states, the development of an antibody that can be used to study DNA damage has numerous applications. In addition to the direct study of DNA damage within cells, this antibody has applications in the development of immunoassays that can monitor 8OHdG excretion in the urine and serve as a biomarker of oxidative stress. Industrial uses may extend to the dietary supplement manufacturers, who could benefit from an immunoassay that could be used to test the effectiveness of antioxidants and other nutraceuticals.

      CHEMICON's new goat antiserum to 8-hydroxyguanosine is now available. This antiserum has been shown by ELISA to be completely cross-reactive with 8-hydroxydeoxyguanosine while not cross-reacting with other naturally occurring nucleotides. This antibody should be a valuable tool for scientists working to understand the role of free radical damage in a number of human disease states.
      Product Information
      HS Code3002 15 90
      • Skin, staining is limited to the nucleus.
      PresentationGoat antiserum. Liquid with 0.1% SODIUM AZIDE.
      Quality LevelMQ100
      ApplicationThis Anti-8-Hydroxydeoxyguanosine Antibody is validated for use in ELISA, IH(P) for the detection of 8-Hydroxydeoxyguanosine.
      Key Applications
      • ELISA
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry: >1:200 on paraffin embedded, formalin fixed human brain. It is suggested that the tissues are pretreated with 10 μg/mL of proteinase K for 40 minutes at 37°C prior to staining.

      Dewaxed sections treated with 3%H2O2 in methanol for 30 minutes and blocking was with 10% NGS in TBS. Sections were treated with proteinase K 10ug/ml in PBS pH 7.4, 40 minutes 37C prior to blocking. Detection is via DAB. Since the antibody is staining a nucleic acid it is important to use enough proteinase to penetrate the histone barrier.
      Optimal working dilutions must be determined by the end user.
      Biological Information
      Immunogen8-Hydroxy-2'-deoxyguanosine and 8-hydroxyguanosine.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityReacts with 8-Hydroxydeoxyguanosine (8-OHdG) and 8-Hydroxyguanosine (8-OHG). By a competitive ELISA the antibody was 100% inhibited by 8-hydroxydeoxyguanosine and 8-hydroxyguanosine nucleotides. Partial cross-reactivity was observed with 8-mercaptoguanosine (80%) while 8-bromoguanosine showed some cross-reactivity (5%). 2-Deoxyadenosine, 7-methlyguanosine, guanosine monophosphate and guanosine showed no reactivity.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Purification MethodUnpurified
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
      Packaging Information
      Material Size200 µL
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Référence GTIN
      AB5830 04053252279546


      Anti-8-Hydroxydeoxyguanosine Antibody FDS


      Fiche de données de sécurité des matériaux (FDS) 

      Anti-8-Hydroxydeoxyguanosine Antibody Certificats d'analyse

      TitreNuméro de lot
      GOAT ANTI- 8-HYDROXYDEOXYGUANOSINE (8-OHdG) - 2947331 2947331
      GOAT ANTI- 8-HYDROXYDEOXYGUANOSINE (8-OHdG) - 3202399 3202399
      GOAT ANTI- 8-HYDROXYDEOXYGUANOSINE (8-OHdG) - 3224644 3224644
      GOAT ANTI- 8-HYDROXYDEOXYGUANOSINE (8-OHdG) - 3286123 3286123
      GOAT ANTI- 8-HYDROXYDEOXYGUANOSINE (8-OHdG) - 3381341 3381341
      GOAT ANTI- 8-HYDROXYDEOXYGUANOSINE (8-OHdG) - 3432599 3432599

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      Stress-induced senescence exaggerates postinjury neointimal formation in the old vasculature.
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      Potassium bromate, a potent DNA oxidizing agent, exacerbates germline repeat expansion in a fragile X premutation mouse model.
      Entezam A, Lokanga AR, Le W, Hoffman G, Usdin K
      Hum Mutat  31  611-6.  2009

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      Loss of fibulin-5 binding to beta1 integrins inhibits tumor growth by increasing the level of ROS.
      Schluterman, Marie K, et al.
      Dis Model Mech  3  333-42  2009

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      Chronic oxidative stress as a mechanism for radiation nephropathy.
      Marek Lenarczyk, Eric P Cohen, Brian L Fish, Amy A Irving, Mukut Sharma, Collin D Driscoll, John E Moulder, Marek Lenarczyk, Eric P Cohen, Brian L Fish, Amy A Irving, Mukut Sharma, Collin D Driscoll, John E Moulder, Marek Lenarczyk, Eric P Cohen, Brian L Fish, Amy A Irving, Mukut Sharma, Collin D Driscoll, John E Moulder, Marek Lenarczyk, Eric P Cohen, Brian L Fish, Amy A Irving, Mukut Sharma, Collin D Driscoll, John E Moulder
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      Pathways and Biomarkers of Oxidative Stress

      Informations techniques

      White Paper- Modern Methods in Oxidative Stress Research

      Fiche technique