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MABE143 Anti-Ago1 Antibody, clone 4G7-E12

Anti-Ago1 Antibody, clone 4G7-E12 is a Mouse Monoclonal Antibody for detection of Ago1 also known as hAgo1, Argonaute1, eIF-2C 1 & has been validated in WB.

Anti-Ago1 Antibody, clone 4G7-E12 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: EIF2C1, AGO1 UniProt Number: Q9UL18
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MABE143
100 µg  
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      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE143
      DescriptionAnti-Ago1 Antibody, clone 4G7-E12
      Alternate Names
      • hAgo1
      • Argonaute1
      • eIF-2C 1
      • eIF2C 1
      • Q99
      • GERP95
      • DKFZp686M13167
      Background InformationAgo1 (Eukaryotic translation initiation factor 2C 1) belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. It is required for G1 arrest and mating in response to nitrogen starvation. It is also known as a cell cycle control protein through its regulation of cytokinesis and its related cell cycle checkpoints, which occur downstream of dcr1. It is required, indirectly, for regulated hyperphosphorylation of cdc2. Ago 1 also has a role in the RNA interference (RNAi) pathway, which is important for heterochromatin formation and accurate chromosome segregation. It has been shown to be non-essential for siRNA-directed target RNA cleavage, but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Ago1 Antibody, clone 4G7-E12 is a Mouse Monoclonal Antibody for detection of Ago1 also known as hAgo1, Argonaute1, eIF-2C 1 & has been validated in WB.
      Key Applications
      • Western Blotting
      Application Notes Immunoprecipitation: A representative lot of this antibody was used for immunoprecipitation by an independent laboratory (see Azuma-Mukai A. et al. (2008) Proc. Natl. Acad. Sci. USA 105: 7964-7969).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human Ago1.
      Clone4G7-E12
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody has been shown by an independent laboratory to not cross react with Ago2, Ago3, or Ago4 (Azuma-Mukai A et al. (2008) Proc. Natl. Acad. Sci. USA 105: 7964-7969).
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and argonaute 4.
      Gene Symbol
      • EIF2C1
      • AGO1
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).

      SUBUNIT STRUCTURE: Interacts with DDB1, DDX6, DDX20, DDX47, DHX9, DICER1, EIF2C2/AGO2, GEMIN4, ILF3, IMP8, MOV10, PRMT5, SART3, TARBP2, TNRC6B and UPF1.

      SUBCELLULAR LOCATION: Cytoplasm > P-body.
      Molecular Weight~97 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected Ago1 in HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABE143 04053252632853

      Documentation

      Anti-Ago1 Antibody, clone 4G7-E12 FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Ago1 Antibody, clone 4G7-E12 Certificats d'analyse

      TitreNuméro de lot
      Anti-Ago1, clone 4G7-E12 3073362
      Anti-Ago1, clone 4G7-E12 - 3744823 3744823
      Anti-Ago1, clone 4G7-E12 - 4123908 4123908
      Anti-Ago1, clone 4G7-E12 - 4153073 4153073
      Anti-Ago1, clone 4G7-E12 - 4270604 4270604
      Anti-Ago1, clone 4G7-E12 -2620668 2620668
      Anti-Ago1, clone 4G7-E12 -2788123 2788123

      Brochure

      Titre
      New Products: Volume 3, 2012

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      Catégories

      Life Science Research > Antibodies and Assays > Primary Antibodies