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ST1546 Anti-CBS Mouse mAb (3E1)

Anti-CBS Mouse mAb (3E1) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-Cystathione Β-Synthase

ST1546
  
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Description
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Recognizes the ~75 kDa CBS protein in HeLa and MCF-7 cells and human hepatocellular carcinoma tissue.
Catalogue NumberST1546
Brand Family Calbiochem®
SynonymsAnti-Cystathione Β-Synthase
Application Data
Detection of human CBS by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-CBS Mouse mAb (3E1) (Cat. No. ST1546) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human CBS by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Primary antibody: Anti-CBS Mouse mAb (3E1) (Cat. No. ST1546) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human CBS by staining paraffin sections. Sample: Formalin-fixed, paraffin embedded human hepatocellular carcinoma tissue. Primary antibody: Anti-CBS Mouse mAb (3E1) (Cat. No. ST1546) (3 µg/ml). Detection: colorimetric (DAB).
References
ReferencesHennig, B., and Diener, M., 2009. Br. J. Pharmacol. 158, 1263.
Lee, M., et al. 2009. Neurobiol. Aging 30, 1523.
Martin, G,R., et al. 2009. Dig. Liver Dis. 42, 103.
Wallace, J,L., et al. 2009. Gastroenterology 137, 569.
Xu, G,Y., et al. 2009. Mol. Pain 5, 44.
Majtan, T., et al. 2008. J. Biol. Chem. 283, 34588.
Wallace, J,L., et al. 2007. FASEB J. 21, 4070.
Schicho, R., et al. 2006. Gastroenterology 131 1542.
Product Information
FormLiquid
FormulationIn PBS, pH 7.2.
Positive controlHeLa cells, MCF-7 cells, Human hepatocellular carcinoma tissue
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Paraffin Sections
Application NotesELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
ImmunogenA recombinant polypeptide corresponding to amino acids 1-101 of human CBS, expressed as a GST fusion protein
Clone3E1
HostMouse
IsotypeIgG2a
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Regulatory Review
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
ST1546 0

Documentation

Références bibliographiques

Aperçu de la référence bibliographique
Hennig, B., and Diener, M., 2009. Br. J. Pharmacol. 158, 1263.
Lee, M., et al. 2009. Neurobiol. Aging 30, 1523.
Martin, G,R., et al. 2009. Dig. Liver Dis. 42, 103.
Wallace, J,L., et al. 2009. Gastroenterology 137, 569.
Xu, G,Y., et al. 2009. Mol. Pain 5, 44.
Majtan, T., et al. 2008. J. Biol. Chem. 283, 34588.
Wallace, J,L., et al. 2007. FASEB J. 21, 4070.
Schicho, R., et al. 2006. Gastroenterology 131 1542.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-November-2010 RFH
SynonymsAnti-Cystathione Β-Synthase
ApplicationELISA (see comments)
Immunoblotting (1-5 µg/ml)
Paraffin Sections (3 µg/ml)
Application Data
Detection of human CBS by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-CBS Mouse mAb (3E1) (Cat. No. ST1546) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human CBS by immunoblotting. Sample: Whole cell lysate from MCF-7 cells. Primary antibody: Anti-CBS Mouse mAb (3E1) (Cat. No. ST1546) (1-5 µg/ml). Detection: chemiluminescence.

Detection of human CBS by staining paraffin sections. Sample: Formalin-fixed, paraffin embedded human hepatocellular carcinoma tissue. Primary antibody: Anti-CBS Mouse mAb (3E1) (Cat. No. ST1546) (3 µg/ml). Detection: colorimetric (DAB).
DescriptionPurified mouse monoclonal antibody. Recognizes the ~75 kDa CBS protein.
BackgroundCBS acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. The encoded protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine β-synthase deficiency (CBSD), which can lead to homocystinuria.
HostMouse
ImmunogenA recombinant polypeptide corresponding to amino acids 1-101 of human CBS, expressed as a GST fusion protein
Clone3E1
IsotypeIgG2a
Specieshuman, rat
Positive controlHeLa cells, MCF-7 cells, Human hepatocellular carcinoma tissue
FormLiquid
FormulationIn PBS, pH 7.2.
CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Regulatory Review
ReferencesHennig, B., and Diener, M., 2009. Br. J. Pharmacol. 158, 1263.
Lee, M., et al. 2009. Neurobiol. Aging 30, 1523.
Martin, G,R., et al. 2009. Dig. Liver Dis. 42, 103.
Wallace, J,L., et al. 2009. Gastroenterology 137, 569.
Xu, G,Y., et al. 2009. Mol. Pain 5, 44.
Majtan, T., et al. 2008. J. Biol. Chem. 283, 34588.
Wallace, J,L., et al. 2007. FASEB J. 21, 4070.
Schicho, R., et al. 2006. Gastroenterology 131 1542.