Millipore Sigma Vibrant Logo

MABE1668 Anti-Cas9 Antibody, CT Antibody, clone 6G12-H11

Anti-Cas9, CT, clone 6G12-H11, Cat. No. MABE1668, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cas9/Csn1 and has been tested in Immunocytochemistry and Western Blotting.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Epigenetics & Nuclear Function Gene Symbol: cas9, csn1 UniProt Number: Q99ZW2
View Products on Sigmaaldrich.com
MABE1668
100 μg  
Prix en cours de récupération
Le prix n'a pas pu être récupéré
La quantité minimale doit être un multiple de
Maximum Quantity is
À la validation de la commande Plus d'informations
Vous avez sauvegardé ()
 
—
Demander le prix
—
Disponibilité limitée Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

      Offres spéciales

       

      Contacter le Service Clients

      Aperçu

      Replacement Information

      Offres spéciales

      100% Performance Guaranteed

      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AICC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1668
      DescriptionAnti-Cas9 Antibody, CT Antibody, clone 6G12-H11
      Alternate Names
      • CRISPR-associated endonuclease Cas9/Csn1
      • SpyCas9
      Background InformationCRISPR-associated endonuclease Cas9/Csn1 (UniProt Q99ZW2; also known as SpyCas9) is encoded by the Streptococcus pyogenes serotype M1 gene Cas9 (also known as csn1) gene (Gene ID 901176). Clustered regularly interspaced short palindromic repeats (CRISPRs) are DNA loci containing short repetitions of base sequences complementary to antecedent mobile elements and target invading nucleic acids. Each repetition is followed by short segments of spacer DNA from previous exposures to a virus. Cas9 is an essential endonuclease in Streptococcus pyogenes serotype M1's CRISPR immune system that confers resistance to foreign genetic elements such as plasmids and phages. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems, correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc), and Cas9. Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, and then trimmed 3'-5' exonucleolytically.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cas9, CT, clone 6G12-H11, Cat. No. MABE1668, is a highly specific mouse monoclonal antibody that targets CRISPR-associated endonuclease Cas9/Csn1 and has been tested in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A 1:50 dilution from a representative lot detected exogenously expressed full-length nuclease-dead Cas9 in transfected NIH/3T3 cells fixed with 4% paraformaldehyde and permeabilized by 0.3% Triton X-100.
      Biological Information
      ImmunogenHis-tagged recombinant Cas9 C-terminal fragment excluding the active sites RuvC-II & RuvC-III.
      Clone6G12-H11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6G12-H11 targets an epitope within Cas9 C-terminal region outside the active sites RuvC-II & RuvC-III.
      IsotypeIgG1κ
      Species Reactivity
      • All
      Species Reactivity NoteStreptococcus pyogenes.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • cas9
      • csn1
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~160 kDa observed. 158.4 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cells.

      Western Blotting Analysis: 0.25 µg/mL of this antibody detected exogenously expressed full-length nuclease-dead Cas9 in 10 µg of lysate from transfected NIH/3T3 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABE1668 04054839108822

      Documentation

      Anti-Cas9 Antibody, CT Antibody, clone 6G12-H11 FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Cas9 Antibody, CT Antibody, clone 6G12-H11 Certificats d'analyse

      TitreNuméro de lot
      Anti-Cas9, CT, clone 6G12-H11 - 3429050 3429050
      Anti-Cas9, CT, clone 6G12-H11 - 3863762 3863762
      Anti-Cas9, CT, clone 6G12-H11 - 3957852 3957852
      Anti-Cas9, CT, clone 6G12-H11 - 3998952 3998952
      Anti-Cas9, CT, clone 6G12-H11 - 4129799 4129799
      Anti-Cas9, CT, clone 6G12-H11 -Q2778167 Q2778167