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AM47 Anti-Caspase-9 (Ab-2) Mouse mAb (1-2)

Anti-Caspase-9 (Ab-2) Mouse mAb (1-2) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-APAF-3, Anti-CASP9, Anti-Mch6, Anti-ICE-LAP6

AM47
  
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Aperçu

Replacement Information

Tableau de caractéristiques principal

Host
M
Description
Overview

This product has been discontinued.





Recognizes the ~46 kDa latent form of caspase-9 in HeLa or Jurkat cells.
Catalogue NumberAM47
Brand Family Calbiochem®
SynonymsAnti-APAF-3, Anti-CASP9, Anti-Mch6, Anti-ICE-LAP6
References
ReferencesCardone, M.H., et al. 1998. Science 282, 1318.
Fearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
Hakem, R., et al. 1998. Cell 94, 339.
Hu, Y., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 4386.
Kuida, K, et al. 1998. Cell 94 325.
Perkins, C., et al. 1998. Cancer Res. 58, 4561.
Duan, H., et al. 1996. J. Biol. Chem. 271, 16720.
Srinivasula, S.M., et al. 1996. J. Biol. Chem. 271, 27099.
Product Information
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Negative controlAnti-trpE (Ab-1) Mouse mAb (I22-25) (Cat. No. OB01)
Positive controlHeLa or Jurkat cells
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Not Frozen Sections
Not Immunofluorescence
Not Immunoprecipitation
Not Paraffin Sections
Application NotesImmunoblotting (1 µg/ml)
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Immunoprecipitation (not recommended)
Paraffin Sections (not recommended)
Application CommentsAntibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena recombinant protein consisting of an amino terminal fragment of human caspase-9
ImmunogenHuman
EpitopeNear the N-terminus
Clone1-2
HostMouse
IsotypeIgG₁
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
AM47 0

Documentation

Anti-Caspase-9 (Ab-2) Mouse mAb (1-2) Certificats d'analyse

TitreNuméro de lot
AM47

Références bibliographiques

Aperçu de la référence bibliographique
Cardone, M.H., et al. 1998. Science 282, 1318.
Fearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
Hakem, R., et al. 1998. Cell 94, 339.
Hu, Y., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 4386.
Kuida, K, et al. 1998. Cell 94 325.
Perkins, C., et al. 1998. Cancer Res. 58, 4561.
Duan, H., et al. 1996. J. Biol. Chem. 271, 16720.
Srinivasula, S.M., et al. 1996. J. Biol. Chem. 271, 27099.

Brochure

Titre
Caspases and other Apoptosis Related Tools Brochure
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-August-2007 RFH
SynonymsAnti-APAF-3, Anti-CASP9, Anti-Mch6, Anti-ICE-LAP6
ApplicationImmunoblotting (1 µg/ml)
Frozen Sections (not recommended)
Immunofluorescence (not recommended)
Immunoprecipitation (not recommended)
Paraffin Sections (not recommended)
DescriptionPurified mouse monoclonal antibody. Recognizes the ~46 kDa latent form of caspase-9.
BackgroundCaspase-9 (ICE-LAP6, MCH-6, APAF-3) is involved in the early activation cascade that occurs following an apoptotic signal. Caspase-9 exists as a pro-form (m.w. aproximately 46 kDa) that is cleaved by the activity of APAF-1 in the presence of cytosolic cytochrome c (APAF-2) and dATP. The processing of pro-caspase-9 into a heterodimer consisting of a 35 kDa and a 10 kDa chain, activates the protease and induces the cascade leading to cleavage of capase-3 and other apoptotic targets. Caspase-9 is also cleaved by that activity of caspase-8 and caspase-10 and also by caspase-3 in a feedback loop. The processing of caspase-9 may be blocked by the activity of bcl-x, which physically interacts with the caspase-9/APAF-1 complex. Processing of caspase-9 is also regulated by the phosphorylation of casapse-9. Mutation of the caspase-9 gene or caspase-9 knockouts results in embryonic lethality due to an enlarged and malformed cerebrum and defective brain development resulting from decreased apoptosis.
HostMouse
Immunogen speciesHuman
Immunogena recombinant protein consisting of an amino terminal fragment of human caspase-9
EpitopeNear the N-terminus
Clone1-2
IsotypeIgG₁
Specieshuman, not mouse, not rat
Positive controlHeLa or Jurkat cells
Negative controlAnti-trpE (Ab-1) Mouse mAb (I22-25) (Cat. No. OB01)
FormLiquid
FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsAntibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesCardone, M.H., et al. 1998. Science 282, 1318.
Fearnhead, H.O., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 13664.
Hakem, R., et al. 1998. Cell 94, 339.
Hu, Y., et al. 1998. Proc. Natl. Acad. Sci. USA 95, 4386.
Kuida, K, et al. 1998. Cell 94 325.
Perkins, C., et al. 1998. Cancer Res. 58, 4561.
Duan, H., et al. 1996. J. Biol. Chem. 271, 16720.
Srinivasula, S.M., et al. 1996. J. Biol. Chem. 271, 27099.