Millipore Sigma Vibrant Logo

PC680 Anti-Caspase-9, Cleaved (Ab-2) Rabbit pAb

PC680
  
Prix en cours de récupération
Le prix n'a pas pu être récupéré
La quantité minimale doit être un multiple de
Maximum Quantity is
À la validation de la commande Plus d'informations
Vous avez sauvegardé ()
 
Demander le prix
Disponibilité limitée
Disponibilité limitée
En stock 
Interrompu(e)
Disponible en quantités limitées
Disponibilité à confirmer
    Pour le restant : Nous vous tiendrons informé
      Pour le restant : Nous vous tiendrons informé
      Nous vous tiendrons informé
      Contacter le Service Clients
      Contact Customer Service

       

      Contacter le Service Clients

      Aperçu

      Replacement Information

      Tableau de caractéristiques principal

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~37 kDa processed form of caspase-9 in actinomycin D induced A431 cells.
      Catalogue NumberPC680
      Brand Family Calbiochem®
      SynonymsAnti-Active Caspase-9
      Application Data
      Detection of human cleaved caspase-9 by immunoblotting. Samples: Whole cell lysate from Jurkat cells left untreated (lane 1) and treated with etoposide (lane 2). Primary antibody: Anti-Caspase-9, Cleaved (Ab-2) Rabbit pAb (Cat. No. PC680) (0.5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol, 1% BSA, pH 7.2.
      Positive controlJurkat cells treated with etoposide
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (1 µg/ml)
      Immunocytochemistry (10-20 µg/ml)
      Application CommentsDoes not recognize full-length caspase-9. Useful for the identification of apoptotic cells in cell populations. To activate caspase-9 treat with an inducer of apoptosis such as actinomycin D at 0.5 µg/ml for 24 h. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids surrounding the Asp³³⁰ cleavage site of human caspase-9
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      PC680 0

      Documentation

      Anti-Caspase-9, Cleaved (Ab-2) Rabbit pAb FDS

      Titre

      Fiche de données de sécurité des matériaux (FDS) 

      Anti-Caspase-9, Cleaved (Ab-2) Rabbit pAb Certificats d'analyse

      TitreNuméro de lot
      PC680

      Brochure

      Titre
      Caspases and other Apoptosis Related Tools Brochure
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-October-2007 RFH
      SynonymsAnti-Active Caspase-9
      ApplicationImmunoblotting (1 µg/ml)
      Immunocytochemistry (10-20 µg/ml)
      Application Data
      Detection of human cleaved caspase-9 by immunoblotting. Samples: Whole cell lysate from Jurkat cells left untreated (lane 1) and treated with etoposide (lane 2). Primary antibody: Anti-Caspase-9, Cleaved (Ab-2) Rabbit pAb (Cat. No. PC680) (0.5 µg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~37 kDa cleaved form of caspase-9.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids surrounding the Asp³³⁰ cleavage site of human caspase-9
      IsotypeIgG
      Specieshuman
      Positive controlJurkat cells treated with etoposide
      FormLiquid
      FormulationIn PBS, 50% glycerol, 1% BSA, pH 7.2.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not recognize full-length caspase-9. Useful for the identification of apoptotic cells in cell populations. To activate caspase-9 treat with an inducer of apoptosis such as actinomycin D at 0.5 µg/ml for 24 h. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling