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MABT917 Anti-Connexin 43 Antibody, clone 2F5

This mouse monoclonal Anti-Connexin 43, clone 2F5, Cat. No. MABT917 is tested for use in Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting for the detection of Connexin-43.

Anti-Connexin 43 Antibody, clone 2F5 : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Cell Structure Gene Symbol: Gja1, Cx43 UniProt Number: P08050
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MABT917
100 μg  
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      Replacement Information

      Tableau de caractéristiques principal

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RIH(P), IF, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT917
      DescriptionAnti-Connexin 43 Antibody, clone 2F5
      Alternate Names
      • Gap junction alpha-1 protein
      • CX43
      • Gap junction 43 kDa heart protein
      Background InformationGap junction alpha-1 protein (UniProt: P08050; also known as Connexin-43, CX43, Gap junction 43 kDa heart protein) is encoded by the Gja1 (also known as Cxn-43) gene (Gene ID: 24392) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 - 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-43 is a multi-pass membrane protein and is the most ubiquitous connexin with widespread tissue expression pattern. Connexin-43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. Phosphorylation at Ser325, Ser328 and Ser330 by casein kinase I is reported to modulate gap junction assembly while phosphorylation of Ser368 by protein kinase C gamma isoform leads to disassembly of gap junction plaques and inhibition of gap junction activity. During myocardial ischemia connexin-43 is shown to become dephosphorylated that allows gap junctions to be opened and convey the propagation of ischemia injury. In bladder smooth muscle cells, connexin-43 displays a circadian cycle with low levels during the sleep phase.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis mouse monoclonal Anti-Connexin 43, clone 2F5, Cat. No. MABT917 is tested for use in Immunohistochemistry (Paraffin), Immunofluorescence, and Western Blotting for the detection of Connexin-43.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunofluorescence
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Connexin 43 in rat heart tissue.

      Western Blotting Analysis: A representative lot detected Connexin 43 in mouse heart tissue lysates, NRK cell lysates, and MDCK cell lysates (Courtesy of Joell Solan in Paul Lampe's Lab at Fred Hutchinson Cancer Research Center, Seattle, WA).

      Immunofluorescence Analysis: A representative lot detected Connexin 43 in NRK cells (Courtesy of Joell Solan in Paul Lampe's Lab at Fred Hutchinson Cancer Research Center, Seattle, WA).
      Biological Information
      ImmunogenA recombinant fragment corresponding to 136 amino acids from the C-terminal half of Rat connexin-43.
      EpitopeC-terminus
      Clone2F5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2F5 detects connexin-43 in human and rat heart. It targets an epitope within 136 amino acids from the C-terminal half.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman, Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Gja1
      • Cx43
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight43.03 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human heart tissue.

      Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Connexin 43 in human heart tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABT917 04054839198960