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MABE2028-100UG Anti-FAN1 Antibody, clone 1A11-2-A

Anti-FAN1, clone 1A11-2-A, Cat. No. MABE2028, is a mouse monoclonal antibody that detects FAN1 and is tested for use in Western Blotting and Proximity Ligation Assay.

MABE2028-100UG
100 μg  
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      Replacement Information
      Description
      Catalogue NumberMABE2028-100UG
      DescriptionAnti-FAN1 Antibody, clone 1A11-2-A
      Alternate Names
      • Fanconi-associated nuclease 1
      • EC:3.1.21
      • FANCD2/FANCI-associated nuclease 1
      • hFAN1
      • Myotubularin-related protein 15
      Background InformationFanconi-associated nuclease 1 (UniProt: Q9Y2M0; also known as EC:3.1.21, FANCD2/FANCI-associated nuclease 1, hFAN1, Myotubularin-related protein 15) is encoded by the FAN1 (also known as KIAA1018, MTMR15) gene (Gene ID: 22909) in human. FAN1 is a nuclease that is required for the repair of DNA interstrand cross-links (ICL). It is recruited to the sites of DNA damage by monoubiquitinated FANCD2, an effector of ATR signaling. It acts as a 5'-3' exonuclease that anchors at a cut end of DNA and cleaves DNA successively at every third nucleotide, allowing to excise an ICL from one strand through flanking incisions. It is specifically involved in repair of ICL-induced DNA breaks and is required for efficient homologous recombination. However, it is not involved in DNA double-strand breaks resection. It is shown to hydrolytically remove 5'-nucleotides successively from the 3'-hydroxy termini of 3'-hydroxy-terminated oligonucleotides. FAN1 is reported to interact with MLH1 via two adjacent MLH1-binding sites, referred to as MLH1-interacting protein (MIP) box and MLH1-interacting motif (MIM). This interaction is regulated during the cell cycle and is inhibited in G2/M phase following CDK1/2-mediated phosphorylation of serine 126 within the MIP box of FAN1. Disruption of the FAN1-MLH1 interaction is reported to cause hypersensitivity to DNA cross-linking agents and defective repair of slipped-CAG/CTG repeats. Two isoforms of FAN1 have been described that are produced by alternative splicing. (Ref.: Porro, A., et al. (2021). , Sci. Adv. 7; eabf7906; Thongthip, S., et al. (2016). Genes & Dev. 2016. 30: 645-659; Wang, R., et al. (2014). Science. 346(6213); 1127-1130; Mackay, C., et al. (2010). Cell. 142(1); 65-76).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-FAN1, clone 1A11-2-A, Cat. No. MABE2028, is a mouse monoclonal antibody that detects FAN1 and is tested for use in Western Blotting and Proximity Ligation Assay.
      Key Applications
      • Western Blotting
      • Proximity Ligation Assay
      Application NotesTested Applications

      Proximity Ligation Assay: A representative lot detected FAN1 in Proximity Ligation Assay (Porro, A., et al. (2021). Sci Adv. 7(31):eabf7906).

      Western Blotting Analysis: A representative lot detected FAN1 in Western Blotting applications (Porro, A., et al. (2021). Sci Adv. 7(31):eabf7906).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenHis-tagged recombinant fragment corresponding to 147 amino acids from the C-terminal region of human Fanconi-associated nuclease 1 (FAN1).
      EpitopeC-terminal
      Clone1A11-2-A
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 1A11-2-A is a mouse monoclonal antibody that detects Fanconi-associated nuclease 1 (FAN1). It targets an epitope within the C-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FAN1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~115 kDa observed; 114.23 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from wild-type HeLa cells.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected FAN1 in lysate from wild-type HeLa cells, but not in lysate form FAN1 knockout HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      MABE2028-100UG 04065270453525