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DR1091 Anti-GATA2 Mouse mAb (2D11)

Anti-GATA2 Mouse mAb (2D11) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Anti-GATA Binding Protein 2

DR1091
  
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      Recognizes the ~50 kDa GATA2 protein in HeLa cells.
      Catalogue NumberDR1091
      Brand Family Calbiochem®
      SynonymsAnti-GATA Binding Protein 2
      Application Data
      Detection of human GATA2 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-GATA2 Mouse mAb (2D11) (Cat. No. DR1091) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human GATA2 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GATA2 Mouse mAb (2D11) (Cat. No. DR1091) (10 µg/ml). Detection: fluorescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHeLa cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 1-103 human GATA2, expressed as a GST fusion protein
      Clone2D11
      HostMouse
      IsotypeIgG2a
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      DR1091 0

      Documentation

      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-November-2010 RFH
      SynonymsAnti-GATA Binding Protein 2
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human GATA2 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-GATA2 Mouse mAb (2D11) (Cat. No. DR1091) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human GATA2 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GATA2 Mouse mAb (2D11) (Cat. No. DR1091) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~50 kDa GATA2 protein.
      BackgroundThis protein is a member of the GATA family of zinc-finger transcription factors that are named for the consensus nucleotide sequence they bind in the promoter regions of target genes. The encoded protein plays an essential role in regulating transcription of genes involved in the development and proliferation of hematopoietic and endocrine cell lineages.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 1-103 human GATA2, expressed as a GST fusion protein
      Clone2D11
      IsotypeIgG2a
      Specieshuman
      Positive controlHeLa cells
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review