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CB1007 Anti-GroEL Mouse mAb (9A1/2)

Anti-GroEL Mouse mAb (9A1/2) : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
CB1007
  
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      Aperçu

      Replacement Information

      Tableau de caractéristiques principal

      Host
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      Description
      Overview

      This product has been discontinued.





      Recognizes the ~60 kDa GroEL protein from E. coli and Pseudomonas.
      Catalogue NumberCB1007
      Brand Family Calbiochem®
      Application Data
      Detection of E. coli GroEL by immunoblotting. Samples: Recombinant GroEL bacterial lysate from protein (lane 1), recombinant Hsp60 protein (negative control) (lane 2), and E. coli (lane 3). Primary antibody: Anti-GroEL Mouse mAb (9A1/2) (Cat. No. CB1007) (1:1000). Detection: chemiluminescence.
      References
      ReferencesDukan, S., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 5746.
      Tang, S.W., et al. 1997. Infect. Immun. 65, 2983.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol.
      Positive controlE. coli lysate
      Preservative≤0.1% sodium azide
      Applications
      Application ReferencesImmunoblotting Dukan, S., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 5746. Immunoprecipitation Tang, S.W., et al. 1997. Infect. Immun. 65, 2983.
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:5000, colorimetric; see application references)
      Immunoprecipitation (1:250; see application references)
      Application CommentsDoes not cross-react with eukaryotic Hsp60, a GroEL homolog. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenGroEL purified from an overproducing strain of E. coli
      ImmunogenE. coli
      Clone9A1/2
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36-A09

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Irritant
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      CB1007 0

      Documentation

      Anti-GroEL Mouse mAb (9A1/2) Certificats d'analyse

      TitreNuméro de lot
      CB1007

      Références bibliographiques

      Aperçu de la référence bibliographique
      Dukan, S., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 5746.
      Tang, S.W., et al. 1997. Infect. Immun. 65, 2983.
      Fiche technique

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-June-2008 JSW
      ApplicationImmunoblotting (1:5000, colorimetric; see application references)
      Immunoprecipitation (1:250; see application references)
      Application Data
      Detection of E. coli GroEL by immunoblotting. Samples: Recombinant GroEL bacterial lysate from protein (lane 1), recombinant Hsp60 protein (negative control) (lane 2), and E. coli (lane 3). Primary antibody: Anti-GroEL Mouse mAb (9A1/2) (Cat. No. CB1007) (1:1000). Detection: chemiluminescence.
      DescriptionMouse monoclonal antibody purified by ammonium sulfate precipitation. Recognizes the ~60 kDa bacterial chaperonin GroEL protein.
      BackgroundGroEL functions in prokaryotic protein folding and assembly in an ATP-dependent manner with another protein of the groE operon, GroES. In vitro, groE gene products have been demonstrated to modulate folding of a number of eukaryotic proteins. GroEL is essential for morphogenesis of bacteriophage lambda, where it mediates assembly of phage head and tail protein complexes. GroEL homologs have been identified in plant chloroplasts and in the mitochondria of a variety of eukaryotes (Hsp60), where in conjunction with Hsp70, they participate in the folding and assembly of proteins translocated into the matrix. Recently a chaperone protein analogous to GroEL was identified in the eukaryotic cytosol, TCP-1, raising the possibility of Hsp60 and Hsp70 cooperation in the maturation of cytosolic proteins.
      HostMouse
      Immunogen speciesE. coli
      ImmunogenGroEL purified from an overproducing strain of E. coli
      Clone9A1/2
      IsotypeIgG₁
      SpeciesE. coli
      Positive controlE. coli lysate
      FormLiquid
      FormulationIn PBS, 50% glycerol.
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with eukaryotic Hsp60, a GroEL homolog. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Irritant
      ReferencesDukan, S., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 5746.
      Tang, S.W., et al. 1997. Infect. Immun. 65, 2983.
      Application referencesImmunoblotting Dukan, S., et al. 2000. Proc. Natl. Acad. Sci. USA 97, 5746. Immunoprecipitation Tang, S.W., et al. 1997. Infect. Immun. 65, 2983.