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ABS1064-I-25UL Anti-Hypusine

Anti-Hypusine Antibody, Cat. No. ABS1064-I, is a rabbit polyclonal antibody that detects Hypusine and has been tested for use in Western Blotting.

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ABS1064-I-25UL
25 µL  
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      Replacement Information
      Description
      Catalogue NumberABS1064-I-25UL
      DescriptionAnti-Hypusine
      Alternate Names
      • N -4-amino-2-hydroxybutyl(lysine)
      Background InformationHypusine (N -4-amino-2-hydroxybutyl(lysine)) is derived by a post-translational modification of lysine. It is found in all eukaryotes and in certain archaea, but not in eubacteria. Currently, eukaryotic translation initiation factor5A (eIF-5A) is the only protein that has been reported to contain hypusine. This modification of elF-5A involves two reactions where in the first reaction deoxyhypusine synthase (DHS) transfers the butylamine moiety of spermidine to the -amino group of a specific lysine residue of eIF-5A resulting in the formation of deoxy-hypusine. This is followed by a hydroxylation reaction catalyzed by deoxyhypusine hydroxylase. This modification appears to be irreversible. The hypusinated form of elF-5A is reported to be the active form that is involved in translational elongation, RNA binding, and ribosome association. Hypusinated eIF5A is shown to be essential for cellular proliferation and responses to extracellular stressors. In the absence of an active hypusine cell proliferation is inhibited and Inhibition of hypusine formation is shown to confer cellular survival in certain stress states, such as infections, carcinogenesis, and obesity. (Ref.: Park, MH., and Wolff, EC. (2018). J. Biol. Chem. 293(48) 18710-18718; Nishiki, Y., et al. (2013). Springerplus. 2;421; Zhai, Q., et al. (2016). J. Mol. Biol. 428(3);603-617).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antiserum with 0.02% sodium azide.
      Applications
      ApplicationAnti-Hypusine Antibody, Cat. No. ABS1064-I, is a rabbit polyclonal antibody that detects Hypusine and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Hypusine in lysate from HEK293 cells transfected with GFP-elF5a wild-type. (Courtesy of Dr. Raghu G Mirmira at Indiana University).
      Biological Information
      ImmunogenSynthetic hypusine-containing peptide C-Ahx-STSKTG[hypusine]HGHAKV-amide.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects hypusinated form of elF-5A.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with all mammals.
      Antibody TypePolyclonal Antibody
      Purification MethodUnpurified
      Molecular Weight~45 kDa observed (GFP-elf-5A). Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysate from HEK293 cells transfected with GFP-elF5a WT.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Hypusine in lysate from HEK293 cells transfected with GFP-elF5a wild-type.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      ABS1064-I-25UL 04061841235996