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ABT61 Anti-INF2 Antibody

Anti-INF2 Antibody is an antibody against INF2 for use in WB, IH(P).

Anti-INF2 Antibody : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Cell Structure Research Sub-Category: Cytoskeletal Signaling Gene Symbol: INF2, C14orf151, C14orf173 UniProt Number: Q27J81
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ABT61
100 µg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT61
      DescriptionAnti-INF2 Antibody
      Alternate Names
      • Inverted formin-2
      • HBEBP2-binding protein C
      Background InformationINF2 (inverted formin 2) is a mouse formin that has been found to interact with actin through the C-terminus to the formin homology 2 domain. The WASP homology 2 motif activates another region that appropriates actin monomers. INF2 has a unique ability to speed up polymerization and depolymerization of actin filaments. Mutations in the N-terminal diaphanous inhibitory domain of INF2 have been proven to be a main cause of familial focal segmental glomerulosclerosis.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HeLa cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-INF2 Antibody is an antibody against INF2 for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:500 dilution from a representative lot of this antibody detected INF2 in stomach cancer tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human INF2.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteDemonstrated to react with Human and Rat.
      Other homologies: Mouse (68% sequence homology).
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySummary: This gene represents a member of the formin family of proteins. It is considered a diaphanous formin due to the presence of a diaphanous inhibitory domain located at the N-terminus of the encoded protein. Studies of a similar mouse protein indicate that the protein encoded by this locus may function in polymerization and depolymerization of actin filaments. Mutations at this locus have been associated with focal segmental glomerulosclerosis 5.
      Gene Symbol
      • INF2
      • C14orf151
      • C14orf173
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Severs actin filaments and accelerates their polymerization and depolymerization (by similarity).

      ENZYME REGULATION: Phosphate inhibits both the depolymerization and severing activities.

      SUBUNIT STRUCTURE: Interacts with actin at the FH2 domain (by similarity).

      SUBCELLULAR LOCATION: Cytoplasm › perinuclear region.

      TISSUE SPECIFICITY: Widely expressed. In the kidney, expression is apparent in podocytes and some tubule cells.

      DOMAIN: The WH2 domain acts as the DAD (diaphanous autoregulatory) domain and binds to actin monomers (by similarity). Regulated by autoinhibition due to intramolecular GBD-DAD binding (by similarity). The severing activity is dependent on covalent attachment of the FH2 domain to the C-terminus (by similarity).

      INVOLVEMENT IN DISEASE: Defects in INF2 are the cause of focal segmental glomerulosclerosis type 5 (FSGS5) [MIM:613237]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.

      SEQUENCE SIMILARITIES: Belongs to the formin homology family. Contains 1 FH2 (formin homology 2) domain. Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain. Contains 1 WH2 domain.

      SEQUENCE CAUTION: The sequence AAH08756.2 differs from that shown. Reason: Erroneous initiation. The sequence AAH64828.1 differs from that shown. Reason: Erroneous initiation. The sequence ABD59343.1 differs from that shown. Reason: Erroneous initiation. The sequence ABD59344.1 differs from that shown. Reason: Erroneous initiation. The sequence ABD59345.1 differs from that shown. Reason: Erroneous initiation. The sequence BAB15224.1 differs from that shown. Reason: Erroneous termination at positions 636 and 759. Translated as Lys, Gln. The sequence EAW81872.1 differs from that shown. Reason: Erroneous gene model prediction.
      Molecular Weight~155 kDa observed.
      INF2 has been shown in WB to migrate as an ~155 kDa band (Madrid, R., et al. (2010). Dev Cell. 18(5):814-827).
      Uniprot describes 3 isoforms produced by alternative splicing with MW's @ ~135, ~134 & ~25 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.05 µg/mL of this antibody detected INF2 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Référence GTIN
      ABT61 04053252293788